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The Crystal Structure of Mlc, a Global Regulator of Sugar Metabolism in Escherichia coli

The Crystal Structure of Mlc, a Global Regulator of Sugar Metabolism in Escherichia coli

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SCHIEFNER, André, Kinga GERBER, Sabine SEITZ, Wolfram WELTE, Kay DIEDERICHS, Winfried BOOS, 2005. The Crystal Structure of Mlc, a Global Regulator of Sugar Metabolism in Escherichia coli. In: The Journal of Biological Chemistry. 280(32), pp. 29073-29079. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M504215200

@article{Schiefner2005Cryst-7456, title={The Crystal Structure of Mlc, a Global Regulator of Sugar Metabolism in Escherichia coli}, year={2005}, doi={10.1074/jbc.M504215200}, number={32}, volume={280}, issn={0021-9258}, journal={The Journal of Biological Chemistry}, pages={29073--29079}, author={Schiefner, André and Gerber, Kinga and Seitz, Sabine and Welte, Wolfram and Diederichs, Kay and Boos, Winfried} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7456"> <dc:format>application/pdf</dc:format> <dcterms:issued>2005</dcterms:issued> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7456/1/72_Schiefner_JBC_280_29073.pdf"/> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7456/1/72_Schiefner_JBC_280_29073.pdf"/> <dc:contributor>Diederichs, Kay</dc:contributor> <dc:creator>Seitz, Sabine</dc:creator> <dcterms:bibliographicCitation>First publ. in: The Journal of Biological Chemistry 280 (2005), 32, pp. 29073-29079</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7456"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:34Z</dc:date> <dc:creator>Boos, Winfried</dc:creator> <dc:creator>Schiefner, André</dc:creator> <dc:contributor>Schiefner, André</dc:contributor> <dcterms:abstract xml:lang="eng">Mlc from Escherichia coli is a transcriptional repressor controlling the expression of a number of genes encoding enzymes of the phosphotransferase system (PTS), including ptsG and manXYZ, the specific enzyme II for glucose and mannose PTS transporters. In addition, Mlc controls the transcription of malT, the gene of the global activator of the mal regulon. The inactivation of Mlc as a repressor is mediated by binding to an actively transporting PtsG (EIICBGlc). Here we report the crystal structure of Mlc at 2.7 Å resolution representing the first described structure of an ROK (repressors, open reading frames, and kinases) family protein. Mlc forms stable dimers thus explaining its binding affinity to palindromic operator sites. The N-terminal helix-turn-helix domain of Mlc is stabilized by the amphipathic C-terminal helix implicated earlier in EIICBGlc binding. Furthermore, the structure revealed a metal-binding site within the cysteine-rich ROK consensus motif that coordinates a structurally important zinc ion. A strongly reduced repressor activity was observed when two of the zinc-coordinating cysteine residues were exchanged against serine or alanine, demonstrating the role of zinc in Mlc-mediated repressor function. The structures of a putative fructokinase from Bacillus subtilis, the glucokinase from Escherichia coli, and a glucomannokinase from Arthrobacter sp. showed high structural homology to the ROK family part of Mlc.</dcterms:abstract> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Welte, Wolfram</dc:contributor> <dc:contributor>Seitz, Sabine</dc:contributor> <dc:contributor>Gerber, Kinga</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:34Z</dcterms:available> <dc:creator>Gerber, Kinga</dc:creator> <dc:creator>Welte, Wolfram</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dcterms:title>The Crystal Structure of Mlc, a Global Regulator of Sugar Metabolism in Escherichia coli</dcterms:title> <dc:creator>Diederichs, Kay</dc:creator> <dc:contributor>Boos, Winfried</dc:contributor> </rdf:Description> </rdf:RDF>

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