Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Seeliger, Sabine; Janssen, Peter H.; Schink, Bernhard

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Dateien

nergetics_and_kinetics_of_lactate_fermentation_to_acetate_and_propionate_via.pdfGröße: 83.57 KBDownloads: 1096

Datum

2002

Autor:innen

Seeliger, Sabine

Janssen, Peter H.

Schink, Bernhard

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

FEMS Microbiology Letters. 2002, 211(1), pp. 65-70. ISSN 0378-1097. Available under: doi: 10.1016/S0378-1097(02)00651-1

Zusammenfassung

Fermentation balances and growth yields were determined with various bacteria fermenting lactate to acetate plus propionate either via methylmalonyl-CoA or via acrylyl-CoA. All strains fermented lactate to acetate plus propionate at approximately a 1:2 ratio. Growth yields of Propionibacterium freudenreichii were more than twice as high as those of Clostridium homopropionicum or Veillonella parvula. Hydrogen was formed as a side product to a significant extent only by V. parvula and Pelobacter propionicus; the latter formed hydrogen preferentially when using ethanol as substrate. Acrylyl-CoA reductase of C. homopropionicum and Clostridium neopropionicum was found nearly exclusively in the cytoplasm thus confirming that this reduction step is unlikely to be involved in energy conservation. C. homopropionicum exhibited higher KS and higher Wmax values, as well as higher specific substrate turnover rates than P. freudenreichii. The results allow us to conclude that C. homopropionicum using the acrylyl-CoA pathway with low growth yield obtains its specific competitive advantage compared to P. freudenreichii not through higher substrate affinity or metabolic shift toward enhanced acetate-plus-hydrogen formation but through faster specific substrate turnover.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Propionate fermentation, methylmalonyl-CoA pathway, acrylyl-CoA pathway, energetics

Zitieren

ISO 690

SEELIGER, Sabine, Peter H. JANSSEN, Bernhard SCHINK, 2002. Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA. In: FEMS Microbiology Letters. 2002, 211(1), pp. 65-70. ISSN 0378-1097. Available under: doi: 10.1016/S0378-1097(02)00651-1

BibTex

@article{Seeliger2002Energ-7451,
  year={2002},
  doi={10.1016/S0378-1097(02)00651-1},
  title={Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA},
  number={1},
  volume={211},
  issn={0378-1097},
  journal={FEMS Microbiology Letters},
  pages={65--70},
  author={Seeliger, Sabine and Janssen, Peter H. and Schink, Bernhard}
}

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