KOPS - Das Institutionelle Repositorium der Universität Konstanz

Tetrahydrobiopterin biosynthesis : Studies with specifically labeled (2H)NAD(P)H and 2H2O and of the enzymes involved

Tetrahydrobiopterin biosynthesis : Studies with specifically labeled (2H)NAD(P)H and 2H2O and of the enzymes involved

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:e42cfc47d41109c8930cd8e75f9821c9

CURTIUS, Hans-Christoph, Dorothee HEINTEL, Sandro GHISLA, Thomas KUSTER, Walter LEIMBACHER, Alois NIEDERWIESER, 1985. Tetrahydrobiopterin biosynthesis : Studies with specifically labeled (2H)NAD(P)H and 2H2O and of the enzymes involved. In: European Journal of Biochemistry. 148(3), pp. 413-419. ISSN 0014-2956. eISSN 1432-1033

@article{Curtius1985Tetra-7427, title={Tetrahydrobiopterin biosynthesis : Studies with specifically labeled (2H)NAD(P)H and 2H2O and of the enzymes involved}, year={1985}, number={3}, volume={148}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={413--419}, author={Curtius, Hans-Christoph and Heintel, Dorothee and Ghisla, Sandro and Kuster, Thomas and Leimbacher, Walter and Niederwieser, Alois} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7427"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:21Z</dc:date> <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 148 (1985), 3, pp. 413-419</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7427"/> <dc:creator>Heintel, Dorothee</dc:creator> <dc:contributor>Curtius, Hans-Christoph</dc:contributor> <dc:creator>Leimbacher, Walter</dc:creator> <dc:creator>Kuster, Thomas</dc:creator> <dcterms:issued>1985</dcterms:issued> <dc:language>eng</dc:language> <dc:creator>Niederwieser, Alois</dc:creator> <dc:contributor>Heintel, Dorothee</dc:contributor> <dcterms:abstract xml:lang="eng">The biosynthesis of tetrahydrobiopterin from either dihydroneopterin triphosphate, sepiapterin, dihydrosepiapterin or dihydrobiopterin was investigated using extracts from human liver, dihydrofolate reductase and purified sepiapterin reductase from human liver and rat erythrocytes. The incorporation of hydrogen in tetrahydrobiopterin was studied in either 2H2O or in H2O using unlabeled NAD(P)H or (R)-(4-2H)NAD(P)H or (S)-(4-2H)NAD(P)H. Dihydrofolate reductase catalyzed the transfer of the pro-R hydrogen of NAD(P)H during the reduction of 7,8-dihydrobiopterin to tetrahydrobiopterin. Sepiapterin reductase catalyzed the transfer of the pro-S hydrogen of NADPH during the reduction of sepiapterin to 7,8-dihydrobiopterin. In the presence of partially purified human liver extracts one hydrogen from the solvent is introduced at position C(6) and the 4-pro-S hydrogen from NADPH is incorporated at each of the C(1') and C(2') position of BH4. Label from the solvent is also introduced into position C(3'). These results suggest that dihydrofolate reductase is not involved in the biosynthesis of tetrahydrobiopterin from dihydroneopterin triphosphate. They are consistent with the assumption of the occurrence of a 6-pyruvoyl-tetrahydropterin intermediate, which is proposed to be formed upon triphosphate elimination from dihydroneopterin triphosphate, and via an intramolecular redox reaction. Our results suggest that the reduction of 6-pyruvoyl-tetrahydropterin might be catalyzed by sepiapterin reductase.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:21Z</dcterms:available> <dc:rights>deposit-license</dc:rights> <dc:contributor>Kuster, Thomas</dc:contributor> <dcterms:title>Tetrahydrobiopterin biosynthesis : Studies with specifically labeled (2H)NAD(P)H and 2H2O and of the enzymes involved</dcterms:title> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:format>application/pdf</dc:format> <dc:contributor>Niederwieser, Alois</dc:contributor> <dc:creator>Ghisla, Sandro</dc:creator> <dc:contributor>Leimbacher, Walter</dc:contributor> <dc:creator>Curtius, Hans-Christoph</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Eur_J_Biochem_1985_CurtiusTetrahydrobiopterin_biosynthesis._Stud.pdf 171

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto