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Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum

Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum

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POLLEGIONI, Loredano, Gaby WELS, Mirella S. PILONE, Sandro GHISLA, 1999. Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum. In: European Journal of Biochemistry. 264(1), pp. 140-151. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1046/j.1432-1327.1999.00586.x

@article{Pollegioni1999Kinet-7405, title={Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum}, year={1999}, doi={10.1046/j.1432-1327.1999.00586.x}, number={1}, volume={264}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={140--151}, author={Pollegioni, Loredano and Wels, Gaby and Pilone, Mirella S. and Ghisla, Sandro} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7405"> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:creator>Pilone, Mirella S.</dc:creator> <dcterms:abstract xml:lang="eng">The kinetic properties of two cholesterol oxidases, one from Brevibacterium sterolicum (BCO) the other from Streptomyces hygroscopicus (SCO) were investigated. BCO works via a ping-pong mechanism, whereas the catalytic pathway of SCO is sequential. The turnover numbers at infinite cholesterol and oxygen concentrations are 202 s−1 and 105 s−1 for SCO and BCO, respectively. The rates of flavin reduction extrapolated to saturating substrate concentration, under anaerobic conditions, are 235 s−1 for BCO and 232 s−1 for SCO (in the presence of 1% Thesit and 10% 2-propanol). With reduced SCO the rate of Δ5-6→Δ4-5 isomerization of the intermediate 5-cholesten-3-one to final product is slow (0.3 s−1). With oxidized SCO and BCO the rate of isomerization is much faster (≈ 300 s−1), thus it is not rate-limiting for catalysis. The kinetic behaviour of both reduced COs towards oxygen is unusual in that they exhibit apparent saturation with increasing oxygen concentrations (extrapolated rates ≈ 250 s−1 and 1.3 s−1, for BCO and SCO, respectively): too slow to account for catalysis. For BCO the kinetic data are compatible with a step preceding the reaction with oxygen, involving interconversion of reactive and nonreactive forms of the enzyme. We suggest that the presence of micelles in the reaction medium, due to the necessary presence of detergents to solubilize the substrate, influence the availability or reactivity of oxygen towards the enzyme. The rate of re-oxidation of SCO in the presence of product is also too slow to account for catalysis, probably due to the impossibility of producing quantitatively the reduced enzyme product complexes.</dcterms:abstract> <dc:creator>Pollegioni, Loredano</dc:creator> <dcterms:issued>1999</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Pilone, Mirella S.</dc:contributor> <dc:rights>deposit-license</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7405/1/Kinetic_mechanisms_of_cholesterol_oxidase_from_Streptomyces_hygroscopicus_and_Brevibacterium_sterolicum.pdf"/> <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 264 (1999), 1, pp. 140-151</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7405"/> <dc:creator>Wels, Gaby</dc:creator> <dc:contributor>Wels, Gaby</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:11Z</dc:date> <dc:contributor>Pollegioni, Loredano</dc:contributor> <dc:format>application/pdf</dc:format> <dc:language>eng</dc:language> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:title>Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum</dcterms:title> <dc:creator>Ghisla, Sandro</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7405/1/Kinetic_mechanisms_of_cholesterol_oxidase_from_Streptomyces_hygroscopicus_and_Brevibacterium_sterolicum.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:11Z</dcterms:available> </rdf:Description> </rdf:RDF>

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