KOPS - Das Institutionelle Repositorium der Universität Konstanz

High-dose erythropoietin alters platelet reactivity and bleeding time in rodents in contrast to the neuroprotective variant carbamyl-erythropoietin (CEPO)

High-dose erythropoietin alters platelet reactivity and bleeding time in rodents in contrast to the neuroprotective variant carbamyl-erythropoietin (CEPO)

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:0c46e4434655b795b7a7513785f6f650

KIRKEBY, Agnete, Lars TORUP, Louise BOCHSEN, Marianne KJALKE, Kristin ABEL, Kim THEILGAARD-MONCH, Pär I. JOHANSSON, Søren E. BJØRN, Jens GERWIEN, Marcel LEIST, 2008. High-dose erythropoietin alters platelet reactivity and bleeding time in rodents in contrast to the neuroprotective variant carbamyl-erythropoietin (CEPO). In: Journal of the International Society on Thrombosis and Haemostasis. 99(4), pp. 720-728. ISSN 0340-6245. Available under: doi: 10.1160/TH07-03-0208

@article{Kirkeby2008Highd-7382, title={High-dose erythropoietin alters platelet reactivity and bleeding time in rodents in contrast to the neuroprotective variant carbamyl-erythropoietin (CEPO)}, year={2008}, doi={10.1160/TH07-03-0208}, number={4}, volume={99}, issn={0340-6245}, journal={Journal of the International Society on Thrombosis and Haemostasis}, pages={720--728}, author={Kirkeby, Agnete and Torup, Lars and Bochsen, Louise and Kjalke, Marianne and Abel, Kristin and Theilgaard-Monch, Kim and Johansson, Pär I. and Bjørn, Søren E. and Gerwien, Jens and Leist, Marcel} }

Torup, Lars High-dose erythropoietin alters platelet reactivity and bleeding time in rodents in contrast to the neuroprotective variant carbamyl-erythropoietin (CEPO) 2008 Kirkeby, Agnete First publ. in: Journal of the International Society on Thrombosis and Haemostasis ; 99 (2008). - pp. 720-728 Kjalke, Marianne Theilgaard-Monch, Kim Bochsen, Louise Leist, Marcel Gerwien, Jens Johansson, Pär I. Leist, Marcel Johansson, Pär I. Gerwien, Jens 2011-03-24T17:34:00Z Abel, Kristin deposit-license Bjørn, Søren E. Kjalke, Marianne application/pdf 2011-03-24T17:34:00Z Bochsen, Louise Abel, Kristin Torup, Lars Bjørn, Søren E. Kirkeby, Agnete Theilgaard-Monch, Kim eng The haematopoietic hormone erythropoietin (EPO) has neuroprotective properties and is currently being explored for treatment of stroke and other neurological disorders. Short-term, high-dose treatment with EPO seems to improve neurological function of stroke patients but may be associated with increased thrombotic risk, whereas alternative non-erythropoietic neuroprotective derivatives of EPO, such as carbamylated EPO (CEPO), may be devoid of such side-effects.We investigated the effects of short-term, high-dose treatment with EPO and CEPO on platelet function and haemostasis in healthy mice and rats. Animals received three daily doses of EPO or CEPO (50 μg/kg), and blood was compared with respect to alterations in haematology and platelet reactivity. In rats, treatment with EPO increased the haematocrit to >50% and the mean platelet volume by 37%,while CEPO had no effect on these parameters.Platelets from EPO-treated rats showed an increased sensitivity to thrombin receptor agonist peptides and elevated plasma levels of soluble P-selectin (sP-selectin) were found in treated mice. Further indicators of platelet hyperreactivity in EPO, but not CEPO-treated animals, were significantly increased aggregatory responses to collagen in whole blood and platelet-rich plasma (PRP).The increased platelet reactivity was paralleled by a decreased bleeding time after tail transection in rats. Samples from EPO-treated rats showed an attenuated response to ADP in whole blood aggregometry and thrombelastography (TEG) platelet mapping but not in apyrase-treated PRP, suggesting involvement of ADP receptor desensitization. These findings suggest that while EPO affects various aspects of platelet function, CEPO is devoid of such effects.

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

kirkeby2008.pdf 90

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto