Oxonol VI as an optical indicator for membrane potentials in lipid vesicles

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APELL, Hans-Jürgen, Beate BERSCH, 1987. Oxonol VI as an optical indicator for membrane potentials in lipid vesicles. In: Biochimica et Biophysica Acta / Biomembranes. 903(3), pp. 480-494. ISSN 0005-2736

@article{Apell1987Oxono-7345, title={Oxonol VI as an optical indicator for membrane potentials in lipid vesicles}, year={1987}, doi={10.1016/0005-2736(87)90055-1}, number={3}, volume={903}, issn={0005-2736}, journal={Biochimica et Biophysica Acta / Biomembranes}, pages={480--494}, author={Apell, Hans-Jürgen and Bersch, Beate} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7345"> <dcterms:issued>1987</dcterms:issued> <dc:format>application/pdf</dc:format> <dc:creator>Bersch, Beate</dc:creator> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dc:contributor>Bersch, Beate</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:42Z</dc:date> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:42Z</dcterms:available> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:abstract xml:lang="eng">Experiments with large unilamellar dioleoylphosphatidylcholine vesicles were carried out in order to study the effect of membrane potential on the fluorescence of Oxonol VI. A partition equilibrium of dye between membrane and water was found to exist with a partition coefficient γtriple bond; length as m-dashclipid/cwater of about 19 000 19000 (at zero voltage). In the presence of an inside-positive membrane potential, the negatively charged dye accumulates in the intravesicular aqueous space according to a Nernst equilibrium. This leads to an increased adsorption of dye to the inner lipid monolayer and to a concomitant increase of fluorescence. The fluorescence change can be calibrated as a function of transmembrane voltage by generating a potassium diffusion potential in the presence of valinomycin. The intrinsic fluorescence of the membrane-bound dye is not affected by voltage; the whole influence of voltage on the fluorescence results from voltage-dependent partitioning of the dye between water and membrane. The voltage dependence of the apparent partition coefficient can be quantitatively described by a three-capacitor model in which the dye is assumed to bind to adsorption planes located on the hydrocarbon side of the membrane / solution interface. Oxonol VI was found to be suitable for detecting changes of membrane potential associated with the activity of the (Na+ + K+)-ATPase in reconstituted vesicles. When ATP is added to the external medium, pump molecules with the ATP-binding side facing outward become activated; this results in a translocation of net positive charge towards the vesicle interior. Under this condition, fluorescence changes corresponding to (inside-positive) potentials of up to 150-200 mV are observed. After the build-up of the membrane potential, a quasi-stationary state is reached in which the pump current is compensated by a back-flow of charge through passive conductance pathways.</dcterms:abstract> <dcterms:bibliographicCitation>First publ. in: Biochimica et Biophysica Acta / Biomembranes, 903 (1987), 3, pp. 480-494</dcterms:bibliographicCitation> <dcterms:title>Oxonol VI as an optical indicator for membrane potentials in lipid vesicles</dcterms:title> <dc:rights>deposit-license</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7345"/> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

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