@article{Anbazhagan2008Incor-7329,
  year={2008},
  doi={10.1021/bi800203g},
  title={Incorporation of Outer Membrane Protein OmpG in Lipid Membranes : Protein-lipid Interactions and β-Barrel Orientation},
  number={23},
  volume={47},
  issn={0006-2960},
  journal={Biochemistry},
  pages={6189--6198},
  author={Anbazhagan, Veerappan and Qu, J. and Kleinschmidt, Jörg and Marsh, Derek}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7329">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:format>application/pdf</dc:format>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7329/1/bi800203g_OmpG_printed_paper.pdf"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7329/1/bi800203g_OmpG_printed_paper.pdf"/>
    <dc:contributor>Anbazhagan, Veerappan</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7329"/>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Qu, J.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Marsh, Derek</dc:creator>
    <dcterms:abstract xml:lang="eng">OmpG is an intermediate size, monomeric, outer membrane protein from Escherichia coli, with nβ ) 14 β-strands. It has a large pore that is amenable to modification by protein engineering. The stoichiometry (Nb ) 20) and selectivity (Kr ) 0.7-1.2) of lipid-protein interaction with OmpG incorporated in dimyristoyl phosphatidylcholine bilayer membranes was determined with various 14-position spinlabeled lipids by using EPR spectroscopy. The limited selectivity for different lipid species is consistent with the disposition of charged residues in the protein. The conformation and orientation (β-strand tilt and β-barrel order parameters) of OmpG in disaturated phosphatidylcholines of odd and even chain lengths from C(12:0) to C(17:0) was determined from polarized infrared spectroscopy of the amide I and amide II bands. A discontinuity in the protein orientation (deduced from the β-barrel order parameters) is observed at the point of hydrophobic matching of the protein with lipid chain length. Compared with smaller (OmpA; nβ ) 8) and larger (FhuA; nβ ) 22) monomeric E. coli outer membrane proteins, the stoichiometry of motionally restricted lipids increases linearly with the number of β-strands, the tilt (β ∼ 44°) of the β-strands is comparable for the three proteins, and the order parameter of the β-barrel increases regularly with nβ. These systematic features of the integration of monomeric β-barrel proteins in lipid membranes could be useful for characterizing outer membrane proteins of unknown structure.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:35Z</dc:date>
    <dcterms:title>Incorporation of Outer Membrane Protein OmpG in Lipid Membranes : Protein-lipid Interactions and β-Barrel Orientation</dcterms:title>
    <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 47 (2008), 23. - S. 6189 6198</dcterms:bibliographicCitation>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:issued>2008</dcterms:issued>
    <dc:creator>Anbazhagan, Veerappan</dc:creator>
    <dc:contributor>Qu, J.</dc:contributor>
    <dc:creator>Kleinschmidt, Jörg</dc:creator>
    <dc:contributor>Marsh, Derek</dc:contributor>
    <dc:contributor>Kleinschmidt, Jörg</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
  </rdf:Description>
</rdf:RDF>