Fermentative degradation of glycolic acid by defined syntrophic cocultures

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1991
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Friedrich, Michael
Laderer, Ute
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Archives of Microbiology. 1991, 156(5), pp. 398-404. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248717
Zusammenfassung

Three different defined cocultures of glycolatedegrading strictly anaerobic bacteria were isolated from enrichment cultures inoculated with freshwater sediment samples. Each culture contained a primary fermenting bacterium which used only glycolate as growth substrate. These cells were gram-positive, formed terminal oval spores, and did not contain cytochromes. Growth with glycolate was possible only in coculture with either a homoacetogenic bacterium or a hydrogen-utilizing methanogenic bacterium; the overall fermentation balance was either 4 glycolate ~3 acetate + 2CO2, or 4 glycolate ~ 3 CH4 + 5 CO2. These transformations indicate that glycolate was converted by the primary fermenting bacterium entirely to CO2 and reducing equivalents which were transferred to the partner organisms, probably through interspecies hydrogen transfer. The key enzymes of fermentative glycolate degradation were identified in cell-free extracts. An acetyl-CoA and ADP-dependent glyoxylate-converting enzyme activity, malic enzyme, pyruvate synthase, and methyl viologendependent hydrogenase were found at comparably high activities suggesting that these bacteria oxidize lycolate through a new pathway via malyl-CoA, and that ATP is synthesized by substrate-level phosphorylation, in a similar manner as found in a recently isolated glyoxylatefermenting anaerobe.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Interspecies hydrogen transfer, Methanogenesis, Homoacetogenesis, Malic enzyme, Substrate level phosphorylation
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Zitieren
ISO 690FRIEDRICH, Michael, Ute LADERER, Bernhard SCHINK, 1991. Fermentative degradation of glycolic acid by defined syntrophic cocultures. In: Archives of Microbiology. 1991, 156(5), pp. 398-404. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248717
BibTex
@article{Friedrich1991Ferme-7318,
  year={1991},
  doi={10.1007/BF00248717},
  title={Fermentative degradation of glycolic acid by defined syntrophic cocultures},
  number={5},
  volume={156},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={398--404},
  author={Friedrich, Michael and Laderer, Ute and Schink, Bernhard}
}
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