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Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici

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ABT, Dietmar J., Oliver EINSLE, Holger NIESSEN, Robert KRIEGER, Albrecht MESSERSCHMIDT, Bernhard SCHINK, Peter M. KRONECK, 2002. Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici. In: Acta Crystallographica. 58(2), pp. 343-345. ISSN 0907-4449. eISSN 1399-0047

@article{Abt2002Cryst-7317, title={Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici}, year={2002}, number={2}, volume={58}, issn={0907-4449}, journal={Acta Crystallographica}, pages={343--345}, author={Abt, Dietmar J. and Einsle, Oliver and Niessen, Holger and Krieger, Robert and Messerschmidt, Albrecht and Schink, Bernhard and Kroneck, Peter M.} }

Abt, Dietmar J. Crystals of the molybdo-/iron±sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N2/H2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water.<br />Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 . Messerschmidt, Albrecht 2011-03-24T17:33:29Z Abt, Dietmar J. eng 2011-03-24T17:33:29Z Krieger, Robert Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici Kroneck, Peter M. Kroneck, Peter M. First publ. in: Acta Crystallographica / Section D, Biological Crystallography, 58 (2002), pp. 343-345 Einsle, Oliver Schink, Bernhard Schink, Bernhard Niessen, Holger Krieger, Robert Niessen, Holger 2002 Messerschmidt, Albrecht Einsle, Oliver terms-of-use application/pdf

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