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Microdomain arrangement of the SERCA-type Ca2+ Pump (Ca2+-ATPase) in subplasmalemmal calcium stores of Paramecium cells

Microdomain arrangement of the SERCA-type Ca2+ Pump (Ca2+-ATPase) in subplasmalemmal calcium stores of Paramecium cells

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PLATTNER, Helmut, Matthias FLÖTENMEYER, Roland KISSMEHL, Nada PAVLOVIC, Karin HAUSER, Massoud MOMAYEZI, N. BRAUN, Jens TACK, Luis BACHMANN, 1999. Microdomain arrangement of the SERCA-type Ca2+ Pump (Ca2+-ATPase) in subplasmalemmal calcium stores of Paramecium cells. In: Journal of Histochemistry and Cytochemistry. 47(7), pp. 841-853. ISSN 0022-1554. eISSN 1551-5044

@article{Plattner1999Micro-7311, title={Microdomain arrangement of the SERCA-type Ca2+ Pump (Ca2+-ATPase) in subplasmalemmal calcium stores of Paramecium cells}, year={1999}, number={7}, volume={47}, issn={0022-1554}, journal={Journal of Histochemistry and Cytochemistry}, pages={841--853}, author={Plattner, Helmut and Flötenmeyer, Matthias and Kissmehl, Roland and Pavlovic, Nada and Hauser, Karin and Momayezi, Massoud and Braun, N. and Tack, Jens and Bachmann, Luis} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7311"> <dc:contributor>Pavlovic, Nada</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Journal of Histochemistry and Cytochemistry ; 47 (1999), 7. - S. 841-853</dcterms:bibliographicCitation> <dc:creator>Hauser, Karin</dc:creator> <dc:creator>Braun, N.</dc:creator> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:title>Microdomain arrangement of the SERCA-type Ca2+ Pump (Ca2+-ATPase) in subplasmalemmal calcium stores of Paramecium cells</dcterms:title> <dc:contributor>Hauser, Karin</dc:contributor> <dc:contributor>Momayezi, Massoud</dc:contributor> <dcterms:issued>1999</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:creator>Pavlovic, Nada</dc:creator> <dc:contributor>Tack, Jens</dc:contributor> <dc:contributor>Flötenmeyer, Matthias</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Bachmann, Luis</dc:creator> <dc:contributor>Kissmehl, Roland</dc:contributor> <dc:creator>Momayezi, Massoud</dc:creator> <dc:contributor>Bachmann, Luis</dc:contributor> <dc:contributor>Braun, N.</dc:contributor> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7311"/> <dc:creator>Flötenmeyer, Matthias</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:26Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:26Z</dc:date> <dcterms:abstract xml:lang="deu">We localized SERCA pumps to the inner region of alveolar sac membranes, facing the cell interior, by combining ultrastructural and biochemical methods. Immunogold labeling largely predominated in the inner alveolar sac region which displayed aggregates of intramembrane particles (IMPs). On image analysis, these represented oligomeric arrangements of ~8-nm large IMP subunits, suggesting formation of SERCA aggregates (as known from sarcoplasmic reticulum). We found not only monomers of typical molecular size (~106 kD) but also oligomeric forms on Western blots (using anti-SERCA antibodies, also against endogenous SERCA from alveolar sacs) and on electrophoresis gelautoradiographs of 32P-labeled phosphoenzyme intermediates. Selective enrichment of SERCA-pump molecules in the inner alveolar sac membrane region may eliminate Ca2+ after centripetal spread observed during exocytosis activation, while the plasmalemmal Ca2+ pump may maintain or reestablish [Ca2+] in the narrow subplasmalemmal space between the outer alveolar sac membrane region and the cell membrane. We show for the first time the microzonal arrangement of SERCA molecules in a Ca2+ store of a secretory system, an intensely discussed issue in stimulus secretion coupling research.</dcterms:abstract> <dc:creator>Kissmehl, Roland</dc:creator> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:creator>Tack, Jens</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> </rdf:Description> </rdf:RDF>

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