Journal article:
Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytes

No Thumbnail Available
Date
2002
Editors
Miller, Robin E.
Fayen, John D.
Mohammad, Shaden F.
Stein, Kevin R.
Daum-Woods, Kathleen
Sramkoski, R. Michael
Jacobberger, James W.
Templeton, Dennis
Shurin, Susan B.
relationships.isEditorOf
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
ArXiv-ID
International patent number
Link to the license
Project
EU project number
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Abstract
Objective: A favorable incidence and severity of graft-vs-host disease is observed in patients transplanted with banked, unrelated, HLA-mismatched umbilical cord blood (UCB) grafts, while the incidence of malignant relapse remains low. CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells.

Materials and Methods: T cells were isolated from UCB and adult peripheral blood and stimulated with anti-CD3 and anti-CD28 monoclonal antibodies. Cells were immunostained and analyzed by flow cytometry for both surface and intracellular expression of CTLA-4 in the presence and absence of cyclosporin A, and kinetics of CTLA-4 expression compared. CTLA-4 mRNA expression was measured using quantitative real-time polymerase chain reaction. NFAT1 protein levels were measured by Western blot analysis.

Results: These studies demonstrate reduced surface and intracellular expression of CTLA-4 in stimulated UCB T cells compared to adult controls. Furthermore, reduced CTLA-4 protein expression in UCB T cells was noted to be in part transcriptionally regulated, as CTLA-4 mRNA levels also were significantly lower. Reduced CLTA-4 expression by UCB T cells followed the kinetics of delayed and reduced expression of the transcription factor NFAT1 by UCB T lymphocytes during primary stimulation. Moreover, cyclosporin A, which is known to modulate NFAT activation, reduced CTLA-4 protein expression in adult and UCB T cells.

Conclusion: Reduced expression of the key regulatory proteins CTLA-4 and NFAT-1 may contribute to favorable UCB T lymphocyte allogeneic responses.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Published in
Experimental Hematology ; 30 (2002), 7. - pp. 738-744. - ISSN 0301-472X
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690MILLER, Robin E., John D. FAYEN, Shaden F. MOHAMMAD, Kevin R. STEIN, Suzanne KADEREIT, Kathleen DAUM-WOODS, R. Michael SRAMKOSKI, James W. JACOBBERGER, Dennis TEMPLETON, Susan B. SHURIN, Mary J. LAUGHLIN, 2002. Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood T lymphocytes. In: Experimental Hematology. 30(7), pp. 738-744. ISSN 0301-472X. Available under: doi: 10.1016/S0301-472X(02)00831-7
BibTex
@article{Miller2002Reduc-7303,
  year={2002},
  doi={10.1016/S0301-472X(02)00831-7},
  title={Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood  T lymphocytes},
  number={7},
  volume={30},
  issn={0301-472X},
  journal={Experimental Hematology},
  pages={738--744},
  author={Miller, Robin E. and Fayen, John D. and Mohammad, Shaden F. and Stein, Kevin R. and Kadereit, Suzanne and Daum-Woods, Kathleen and Sramkoski, R. Michael and Jacobberger, James W. and Templeton, Dennis and Shurin, Susan B. and Laughlin, Mary J.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7303">
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:issued>2002</dcterms:issued>
    <dc:creator>Miller, Robin E.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Stein, Kevin R.</dc:creator>
    <dc:creator>Laughlin, Mary J.</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7303"/>
    <dc:creator>Shurin, Susan B.</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <dc:language>deu</dc:language>
    <dc:contributor>Kadereit, Suzanne</dc:contributor>
    <dc:creator>Kadereit, Suzanne</dc:creator>
    <dc:contributor>Miller, Robin E.</dc:contributor>
    <dc:creator>Jacobberger, James W.</dc:creator>
    <dc:contributor>Jacobberger, James W.</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:23Z</dcterms:available>
    <dcterms:bibliographicCitation>First publ. in: Experimental Hematology 30 (2002), 7, pp. 738-744</dcterms:bibliographicCitation>
    <dc:creator>Fayen, John D.</dc:creator>
    <dc:creator>Mohammad, Shaden F.</dc:creator>
    <dc:contributor>Sramkoski, R. Michael</dc:contributor>
    <dc:creator>Daum-Woods, Kathleen</dc:creator>
    <dc:contributor>Templeton, Dennis</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7303/1/Miller_et_al._2002.pdf"/>
    <dc:contributor>Daum-Woods, Kathleen</dc:contributor>
    <dc:creator>Templeton, Dennis</dc:creator>
    <dcterms:abstract xml:lang="eng">Objective: A favorable incidence and severity of graft-vs-host disease is observed in patients transplanted with banked, unrelated, HLA-mismatched umbilical cord blood (UCB) grafts, while the incidence of malignant relapse remains low. CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells.&lt;br /&gt;&lt;br /&gt;Materials and Methods: T cells were isolated from UCB and adult peripheral blood and stimulated with anti-CD3 and anti-CD28 monoclonal antibodies. Cells were immunostained and analyzed by flow cytometry for both surface and intracellular expression of CTLA-4 in the presence and absence of cyclosporin A, and kinetics of CTLA-4 expression compared. CTLA-4 mRNA expression was measured using quantitative real-time polymerase chain reaction. NFAT1 protein levels were measured by Western blot analysis.&lt;br /&gt;&lt;br /&gt;Results: These studies demonstrate reduced surface and intracellular expression of CTLA-4 in stimulated UCB T cells compared to adult controls. Furthermore, reduced CTLA-4 protein expression in UCB T cells was noted to be in part transcriptionally regulated, as CTLA-4 mRNA levels also were significantly lower. Reduced CLTA-4 expression by UCB T cells followed the kinetics of delayed and reduced expression of the transcription factor NFAT1 by UCB T lymphocytes during primary stimulation. Moreover, cyclosporin A, which is known to modulate NFAT activation, reduced CTLA-4 protein expression in adult and UCB T cells.&lt;br /&gt;&lt;br /&gt;Conclusion: Reduced expression of the key regulatory proteins CTLA-4 and NFAT-1 may contribute to favorable UCB T lymphocyte allogeneic responses.</dcterms:abstract>
    <dc:contributor>Mohammad, Shaden F.</dc:contributor>
    <dc:contributor>Stein, Kevin R.</dc:contributor>
    <dc:contributor>Shurin, Susan B.</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7303/1/Miller_et_al._2002.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:23Z</dc:date>
    <dc:contributor>Laughlin, Mary J.</dc:contributor>
    <dcterms:title>Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood  T lymphocytes</dcterms:title>
    <dc:creator>Sramkoski, R. Michael</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Fayen, John D.</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed
Link to research data
Description of supplementary data