Freeze etching of cells without cryoprotectants

Cite This

Files in this item

Checksum: MD5:88363c7ef74b80f267eb6014530540e8

PLATTNER, Helmut, Walter M. FISCHER, Werner W. SCHMITT, Luis BACHMANN, 1972. Freeze etching of cells without cryoprotectants. In: Journal of Cell Biology. 53(1), pp. 116-126. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.53.1.116

@article{Plattner1972Freez-7259, title={Freeze etching of cells without cryoprotectants}, year={1972}, doi={10.1083/jcb.53.1.116}, number={1}, volume={53}, issn={0021-9525}, journal={Journal of Cell Biology}, pages={116--126}, author={Plattner, Helmut and Fischer, Walter M. and Schmitt, Werner W. and Bachmann, Luis} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7259"> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7259/1/Freeze_etching_of_cells_without_cryoprotectants.pdf"/> <dc:contributor>Fischer, Walter M.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Plattner, Helmut</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:creator>Bachmann, Luis</dc:creator> <dc:contributor>Bachmann, Luis</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Journal of Cell Biology 53 (1972), pp. 116-126</dcterms:bibliographicCitation> <dc:rights>terms-of-use</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:02Z</dcterms:available> <dcterms:title>Freeze etching of cells without cryoprotectants</dcterms:title> <dcterms:issued>1972</dcterms:issued> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7259"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7259/1/Freeze_etching_of_cells_without_cryoprotectants.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Schmitt, Werner W.</dc:contributor> <dc:creator>Fischer, Walter M.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:02Z</dc:date> <dc:creator>Schmitt, Werner W.</dc:creator> <dcterms:abstract xml:lang="eng">The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.</dcterms:abstract> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Freeze_etching_of_cells_without_cryoprotectants.pdf 307

This item appears in the following Collection(s)

terms-of-use Except where otherwise noted, this item's license is described as terms-of-use

Search KOPS


Browse

My Account