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Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine

Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine

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MIKHAILOV, Andrey, Ann-Sofi HÄRMÄLÄ-BRASKÉN, Jussi MERILUOTO, Yulia SOROKINA, Daniel R. DIETRICH, John E. ERIKSSON, 2001. Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine. In: Toxicon. 39(4), pp. 477-483. ISSN 0041-0101

@article{Mikhailov2001Produ-7217, title={Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine}, year={2001}, doi={10.1016/S0041-0101(00)00148-3}, number={4}, volume={39}, issn={0041-0101}, journal={Toxicon}, pages={477--483}, author={Mikhailov, Andrey and Härmälä-Braskén, Ann-Sofi and Meriluoto, Jussi and Sorokina, Yulia and Dietrich, Daniel R. and Eriksson, John E.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7217"> <dc:creator>Dietrich, Daniel R.</dc:creator> <dc:contributor>Härmälä-Braskén, Ann-Sofi</dc:contributor> <dcterms:issued>2001</dcterms:issued> <dcterms:title>Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine</dcterms:title> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7217"/> <dcterms:abstract xml:lang="eng">Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue green algae). Their toxicity is associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). We have developed a battery of antibodies to microcystins using chemical modification (aminoethylation) of one of its core amino acids, N-methyl-dehydroalanine. The developed antibodies displayed different reactivities to closely related MCs. Selected monoclonal antibodies were used for quantitative competitive ELISA assays. The analytical sensitivity of these assays was up to 1 ng/ml. Comparison of the developed ELISA tests with HPLC-based measurements of MCs in laboratory and field samples showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide the means for developing extremely sensitive and specific analytical assays for direct measurement of toxins in cyanobacterial or water samples.</dcterms:abstract> <dc:creator>Härmälä-Braskén, Ann-Sofi</dc:creator> <dcterms:bibliographicCitation>First publ. in: Toxicon 39 (2001), 4, pp. 477-483</dcterms:bibliographicCitation> <dc:creator>Mikhailov, Andrey</dc:creator> <dc:contributor>Mikhailov, Andrey</dc:contributor> <dc:contributor>Eriksson, John E.</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:creator>Eriksson, John E.</dc:creator> <dc:format>application/pdf</dc:format> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:creator>Meriluoto, Jussi</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:43Z</dc:date> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:43Z</dcterms:available> <dc:contributor>Dietrich, Daniel R.</dc:contributor> <dc:contributor>Meriluoto, Jussi</dc:contributor> <dc:creator>Sorokina, Yulia</dc:creator> <dc:contributor>Sorokina, Yulia</dc:contributor> </rdf:Description> </rdf:RDF>

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