Production and characterization of monoclonal antibodies against ochratoxin B

Heussner, Alexandra H.; Möller, Ines; Day, Billy W.; Dietrich, Daniel R.; O'Brien, Evelyn

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Production and characterization of monoclonal antibodies against ochratoxin B

Type of Publication:	Journal article
URI (citable link):	http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-49867
Author:	Heussner, Alexandra H.; Möller, Ines; Day, Billy W.; Dietrich, Daniel R.; O'Brien, Evelyn
Year of publication:	2007
Published in:	Food and Chemical Toxicology ; 45 (2007), 5. - pp. 827-833. - ISSN 0278-6915. - eISSN 1873-6351
Summary:	Monoclonal antibodies against ochratoxin B (OTB) were generated by immunizing Balb/c mice with OTB conjugated to keyhole limpet hemocyanin (KLH) via carbodiimide reactions with CHMC and EDAC. A stable hybridoma cell line 2F1.E10 was produced by fusion of murine splenocytes and myeloma cells. The obtained antibodies were characterized using an indirect competitive ELISA. The detection limit was calculated (27 ± 2 nM OTB) and 50% binding inhibition was reached at 500 nM free OTB. A low cross-reactivity to ochratoxin A (OTA) of 3.3% and no cross-reactivities to either coumarin or dl-phenylalanine were observed, suggesting a highly specific OTB antibody. The antibody type was identified as IgG class 1 with the light chain being of the κ configuration. These antibodies can be used in an indirect competitive ELISA to detect OTB in the nanomolar to micromolar concentration range and may be useful for the analysis of contaminated food items.
Subject (DDC):	570 Biosciences, Biology
Keywords:	Ochratoxin B, Monoclonal antibodies, ELISA
Link to License:	Terms of use
Bibliography of Konstanz:	Yes

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HEUSSNER, Alexandra H., Ines MÖLLER, Billy W. DAY, Daniel R. DIETRICH, Evelyn O'BRIEN, 2007. Production and characterization of monoclonal antibodies against ochratoxin B. In: Food and Chemical Toxicology. 45(5), pp. 827-833. ISSN 0278-6915. eISSN 1873-6351

@article{Heussner2007Produ-7164, title={Production and characterization of monoclonal antibodies against ochratoxin B}, year={2007}, number={5}, volume={45}, issn={0278-6915}, journal={Food and Chemical Toxicology}, pages={827--833}, author={Heussner, Alexandra H. and Möller, Ines and Day, Billy W. and Dietrich, Daniel R. and O'Brien, Evelyn} }

Heussner, Alexandra H. Heussner, Alexandra H. Production and characterization of monoclonal antibodies against ochratoxin B Monoclonal antibodies against ochratoxin B (OTB) were generated by immunizing Balb/c mice with OTB conjugated to keyhole limpet hemocyanin (KLH) via carbodiimide reactions with CHMC and EDAC. A stable hybridoma cell line 2F1.E10 was produced by fusion of murine splenocytes and myeloma cells. The obtained antibodies were characterized using an indirect competitive ELISA. The detection limit was calculated (27 ± 2 nM OTB) and 50% binding inhibition was reached at 500 nM free OTB. A low cross-reactivity to ochratoxin A (OTA) of 3.3% and no cross-reactivities to either coumarin or dl-phenylalanine were observed, suggesting a highly specific OTB antibody. The antibody type was identified as IgG class 1 with the light chain being of the κ configuration.<br />These antibodies can be used in an indirect competitive ELISA to detect OTB in the nanomolar to micromolar concentration range and may be useful for the analysis of contaminated food items. eng Day, Billy W. Dietrich, Daniel R. O'Brien, Evelyn First publ. in: Food and Chemical Toxicology 45 (2007), 5, pp. 827-833 Möller, Ines Dietrich, Daniel R. Möller, Ines 2011-03-24T17:32:21Z 2007 O'Brien, Evelyn 2011-03-24T17:32:21Z terms-of-use application/pdf Day, Billy W.