KOPS - Das Institutionelle Repositorium der Universität Konstanz

The structure of the covalent flavin adduct formed between lactate oxidase and the suicide substrate 2-hydroxy-3-butynoate

The structure of the covalent flavin adduct formed between lactate oxidase and the suicide substrate 2-hydroxy-3-butynoate

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:4b0528a35beddb9a7f9e13dd92af5f55

SCHONBRUNN, Agnes, Robert H. ABELES, Christopher T. WALSH, Sandro GHISLA, Hatenori OGATA, Vincent MASSEY, 1976. The structure of the covalent flavin adduct formed between lactate oxidase and the suicide substrate 2-hydroxy-3-butynoate. In: Biochemistry. 15(9), pp. 1798-1807. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi00654a003

@article{Schonbrunn1976struc-7158, title={The structure of the covalent flavin adduct formed between lactate oxidase and the suicide substrate 2-hydroxy-3-butynoate}, year={1976}, doi={10.1021/bi00654a003}, number={9}, volume={15}, issn={0006-2960}, journal={Biochemistry}, pages={1798--1807}, author={Schonbrunn, Agnes and Abeles, Robert H. and Walsh, Christopher T. and Ghisla, Sandro and Ogata, Hatenori and Massey, Vincent} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7158"> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Schonbrunn, Agnes</dc:contributor> <dc:contributor>Massey, Vincent</dc:contributor> <dc:creator>Schonbrunn, Agnes</dc:creator> <dcterms:title>The structure of the covalent flavin adduct formed between lactate oxidase and the suicide substrate 2-hydroxy-3-butynoate</dcterms:title> <dc:format>application/pdf</dc:format> <dc:contributor>Ghisla, Sandro</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7158/1/Biochemistry_1976_SchonbrunnThe_structure_of_the_covalent.pdf"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Abeles, Robert H.</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">2-Hydroxy-3-butynoic acid is a suicide substrate for Mycobacterium smegmatis lactate oxidase. Inactivation occurs by covalent modification of enzyme-bound FMN and does not involve labeling of the apoprotein. The spectrum of the enzyme bound adduct suggests that it is a 4a,5-dihydroflavin derivative. When this adduct is released from the enzyme, a complex mixture of unstable compounds is obtained. When the initially formed enzyme-bound adduct is reduced with NaBH4, a major stable species can be resolved from the enzyme and can be isolated and purified. The structure was established by appropriate isotope substitutions, Fourier transform NMR spectroscopy, chemical reactivity, and synthesis of a model compound. The structure of the isolated adduct is structure 11, Scheme 11. The structure proposed for the adduct initially formed on the enzyme is structure VII, Scheme 11.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:18Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Massey, Vincent</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:contributor>Ogata, Hatenori</dc:contributor> <dc:contributor>Walsh, Christopher T.</dc:contributor> <dc:contributor>Abeles, Robert H.</dc:contributor> <dc:creator>Ghisla, Sandro</dc:creator> <dc:creator>Ogata, Hatenori</dc:creator> <dc:creator>Walsh, Christopher T.</dc:creator> <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 15 (1976), 9. - S. 1798-1807</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7158"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7158/1/Biochemistry_1976_SchonbrunnThe_structure_of_the_covalent.pdf"/> <dcterms:issued>1976</dcterms:issued> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Biochemistry_1976_SchonbrunnThe_structure_of_the_covalent.pdf 162

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto