SP-A Binding Sites on Bovine Alveolar Macrophages

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PLAGA, Sandra, Helmut PLATTNER, Jutta SCHLEPPER-SCHAEFER, 1998. SP-A Binding Sites on Bovine Alveolar Macrophages. In: Experimental Cell Research. 245(1), pp. 116-122. ISSN 0014-4827

@article{Plaga1998Bindi-7154, title={SP-A Binding Sites on Bovine Alveolar Macrophages}, year={1998}, doi={10.1006/excr.1998.4226}, number={1}, volume={245}, issn={0014-4827}, journal={Experimental Cell Research}, pages={116--122}, author={Plaga, Sandra and Plattner, Helmut and Schlepper-Schaefer, Jutta} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7154"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:16Z</dc:date> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:title>SP-A Binding Sites on Bovine Alveolar Macrophages</dcterms:title> <dc:contributor>Schlepper-Schaefer, Jutta</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Experimental Cell Research 245 (1998), 1, pp. 116-122</dcterms:bibliographicCitation> <dc:rights>deposit-license</dc:rights> <dcterms:abstract xml:lang="eng">Surfactant protein A (SP-A) binding to bovine alveolar macrophages was examined in order to characterize SP-A binding proteins on the cell surface and to isolate putative receptors from these cells that could be obtained in large amounts. Human SP-A, unlabeled or labeled with gold particles, was bound to freshly isolated macrophages and analyzed with ELISA or the transmission electron microscope. Binding of SP-A was inhibited by Ca2+chelation, by an excess of unlabeled SP-A, or by the presence of 20 mg/ml mannan. We conclude that bovine alveolar macrophages expose binding sites for SP-A that are specific and that depend on Ca2+and on mannose residues. For isolation of SP-A receptors with homologous SP-A as ligand we isolated SP-A from bovine lung lavage. SDS PAGE analysis of the purified SP-A showed a protein of 32 36 kDa. Functional integrity of the protein was demonstrated. Bovine SP-A bound to Dynabeads was used to isolate SP-A binding proteins. From the fractionated and blotted proteins of the receptor preparation two proteins bound SP-A in a Ca2+-dependent manner, a 40-kDa protein showing mannose dependency and a 210-kDa protein, showing no mannose sensitivity.</dcterms:abstract> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Plaga, Sandra</dc:contributor> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:creator>Plaga, Sandra</dc:creator> <dcterms:issued>1998</dcterms:issued> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7154"/> <dc:creator>Schlepper-Schaefer, Jutta</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:16Z</dcterms:available> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

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