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Determination of the eicosanoid response to inflammatory stimuli in whole blood and its pharmacological modulation ex vivo

Determination of the eicosanoid response to inflammatory stimuli in whole blood and its pharmacological modulation ex vivo

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AULOCK, Sonja von, Corinna HERMANN, Thomas HARTUNG, 2003. Determination of the eicosanoid response to inflammatory stimuli in whole blood and its pharmacological modulation ex vivo. In: Journal of immunological methods. 277(1-2), pp. 53-63. ISSN 0022-1759

@article{Aulock2003Deter-7150, title={Determination of the eicosanoid response to inflammatory stimuli in whole blood and its pharmacological modulation ex vivo}, year={2003}, doi={10.1016/S0022-1759(03)00180-7}, number={1-2}, volume={277}, issn={0022-1759}, journal={Journal of immunological methods}, pages={53--63}, author={Aulock, Sonja von and Hermann, Corinna and Hartung, Thomas} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7150"> <dc:contributor>Aulock, Sonja von</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:issued>2003</dcterms:issued> <dc:contributor>Hermann, Corinna</dc:contributor> <dc:contributor>Hartung, Thomas</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:title>Determination of the eicosanoid response to inflammatory stimuli in whole blood and its pharmacological modulation ex vivo</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:15Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:32:15Z</dc:date> <dcterms:bibliographicCitation>First publ. in: Journal of immunological methods 277 (2003), 1-2, pp. 53-63</dcterms:bibliographicCitation> <dcterms:abstract xml:lang="eng">Recognition of pathogens by immune cells initiates the release of numerous signaling molecules, including cytokines and eicosanoids. Here, we describe a simple procedure by which eicosanoids such as prostaglandin E2 (PGE2), leukotriene B4 (LTB4) and thromboxane B2 (TxB2) can be measured using commercial enzyme immunoassays (EIAs) in the supernatant of whole blood stimulated with inflammatory stimuli. This is illustrated for numerous stimuli. The kinetics by which lipopolysaccharide (LPS) induces cyclooxygenase (COX)-2 expression in this setup were determined by quantitative reverse transcription polymerase chain reaction (RT-PCR). The eicosanoid response of the blood of 160 healthy volunteers to 1 μg/ml LPS was measured. To determine whether the action of a drug in vivo is represented ex vivo in the eicosanoid response of blood, one volunteer took a standard dose of a number of commercially available cyclooxygenase inhibitors on different days and the eicosanoid response of his blood to LPS was determined before ingestion as well as 2 and 6 h afterwards. The efficacy of the different pharmaceuticals on cyclooxygenase but not lipoxygenase products or cytokines could be monitored ex vivo. Similarly, ex vivo eicosanoid release was measured in blood from 10 volunteers who had taken 50 mg flurbiprofen. The method described extends approaches for studying whole blood cytokine release to the lipid mediators formed from arachidonic acid. These important signaling molecules represent targets for pharmacological intervention, which can now be monitored in vitro, as well as ex vivo employing the same model. Furthermore, the assay could be used to characterize the immune status of patient groups or to monitor the course of disease.</dcterms:abstract> <dc:creator>Hermann, Corinna</dc:creator> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7150"/> <dc:creator>Hartung, Thomas</dc:creator> <dc:creator>Aulock, Sonja von</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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