Syntrophe Oxidation der Fettsäure Acetat und des biogenen Amins Cadaverin (1,5-Diaminopentan) durch definierte methanogene Kokulturen

Röder, Julia Christine

Syntrophe Oxidation der Fettsäure Acetat und des biogenen Amins Cadaverin (1,5-Diaminopentan) durch definierte methanogene Kokulturen

Files

Diss_Roeder.pdf(5.5 MB)

Date

2010

Authors

Röder, Julia Christine

Title in another language

Syntrophic oxidation of the fatty acid acetate and the biogenic amine cadaverine (1,5-diaminopentane) by defined methanogenic cocultures

Publication type

Dissertation

Abstract

In this work the anaerobic syntrohic oxidation of the fatty acid acetate and the biogenic amine cadaverine (1,5-diaminopentane) by two different defined cocultures was investigated. The manner of the anaerobic oxidation of the fatty acid acetate by the thermophilic coculture Synac should be clarified. Therefore the acetate fermenting bacterium Thermacetogenium phaeum was tested for the presence of the key enzymes of the Wood-Ljungdahl pathway in pure culture and comparatively in syntrophic grown cells. Distinctions of the enzymatic expression under both growing circumstances were investigated and depicted biochemically and proteomically. Proteins were sorted into cytoplasmic and membrane associated, or membrane integral proteins, respectively according to their distribution in the fractions. Based on this specific composition, conclusions about the possible energy gaining steps of the acetate degradation were drawn. A possible sodium dependance of the acetogenic bacterium T. phaeum should be investigated. The mesophilic coculture LC 13M, isolated from the enrichment culture La Cad, should be characterized physiologically and phylogenetically. The pathway of the cadaverine degradation should be mapped applying biochemical methods. The thereby forming products should be determined and quantified.

Summary in another language

In dieser Arbeit wurde die anaerobe, syntrophe Oxidation der Fettsäure Acetat und des biogenen Amins Cadaverin (1,5-Diaminopentan) durch zwei verschiedene definierte Kokulturen untersucht. Es sollte geklärt werden auf welche Weise die syntrophe thermophile Kokultur Synac die Fettsäure Acetat anaerob oxidiert. Dazu sollte das Acetat vergärende Bakterium Thermacetogenium phaeum einerseits in Reinkultur, andererseits syntroph gewachsen - auf das Vorhandensein der Schlüsselenzyme des Wood-Ljungdahl Abbauweges untersucht werden. Unterschiede in der Enzymexpression unter beiden Wachstumsbedingungen sollten biochemisch und proteomisch untersucht und dargestellt werden. Die Proteine sollten bezüglich ihrer Lokalisation in den Kompartimenten in cytoplasmatische, oder Membran-assoziierte, bzw. integrierte Proteine eingeordnet werden. Aufgrund dieser spezifischen Anordnung sollten Rückschlüsse auf die möglichen energieliefernden Schritte des Acetatabbaus gezogen werden. Eine eventuelle Natriumabhängigkeit des acetogenen Bakteriums T. phaeum sollte untersucht werden.
Die aus der Anreicherungskultur La Cad isolierte mesophile Kokultur LC 13M sollte physiologisch und phylogenetisch charakterisiert werden. Es sollte der Weg des Cadaverinabbaus durch biochemische Methoden aufgeschlüsselt werden. Die sich dabei bildenden Produkte sollten bestimmt und quantifiziert werden.

Subject (DDC)

500 Natural Sciences

Keywords

Syntrophe Acetat-Oxidation,anaerob,Acetogene Bakterien,Wood-Ljungdahl-Weg,biogenes Amin,Cadaverin,syntrophic acetate oxidation,anaerobic,acetogenic bacteria,Wood-Ljungdahl pathway,biogenic amine,cadaverine

Cite This

ISO 690

RÖDER, Julia Christine, 2010. Syntrophe Oxidation der Fettsäure Acetat und des biogenen Amins Cadaverin (1,5-Diaminopentan) durch definierte methanogene Kokulturen [Dissertation]. Konstanz: University of Konstanz

BibTex

@phdthesis{Roder2010Syntr-7129,
  year={2010},
  title={Syntrophe Oxidation der Fettsäure Acetat und des biogenen Amins Cadaverin (1,5-Diaminopentan) durch definierte methanogene Kokulturen},
  author={Röder, Julia Christine},
  address={Konstanz},
  school={Universität Konstanz}
}

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Examination date of dissertation

September 10, 2010