Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können kommenden Montag und Dienstag keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted next Monday and Tuesday.)
Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-56646 |
Author: | Macheroux, Peter; Schmidt, K. U.; Steinerstauch, Petra; Ghisla, Sandro; Colepicolo, Pio; Buntic, Rudolf; Hastings, J. Woodland |
Year of publication: | 1987 |
Published in: | Biochemical and Biophysical Research Communications ; 146 (1987), 1. - pp. 101-106. - ISSN 0006-291X |
Summary: |
A low molecular weight protein (~ 25,000 D) exhibiting a yellow fluorescence emission peaking at ~ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λmax = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (λmax ~ 495 nm), this protein induces a bright yellow luminescence (λmax ~ 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.
|
Subject (DDC): | 570 Biosciences, Biology |
Link to License: | Attribution-NonCommercial-NoDerivs 2.0 Generic |
MACHEROUX, Peter, K. U. SCHMIDT, Petra STEINERSTAUCH, Sandro GHISLA, Pio COLEPICOLO, Rudolf BUNTIC, J. Woodland HASTINGS, 1987. Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore. In: Biochemical and Biophysical Research Communications. 146(1), pp. 101-106. ISSN 0006-291X
@article{Macheroux1987Purif-7105, title={Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore}, year={1987}, number={1}, volume={146}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={101--106}, author={Macheroux, Peter and Schmidt, K. U. and Steinerstauch, Petra and Ghisla, Sandro and Colepicolo, Pio and Buntic, Rudolf and Hastings, J. Woodland} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7105"> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:format>application/pdf</dc:format> <dc:contributor>Colepicolo, Pio</dc:contributor> <dc:contributor>Steinerstauch, Petra</dc:contributor> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:32Z</dc:date> <dc:creator>Buntic, Rudolf</dc:creator> <dc:creator>Colepicolo, Pio</dc:creator> <dc:contributor>Macheroux, Peter</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7105"/> <dc:creator>Hastings, J. Woodland</dc:creator> <dc:creator>Steinerstauch, Petra</dc:creator> <dcterms:bibliographicCitation>First publ. in: Biochemical and Biophysical Research Communications 146 (1987), 1, pp. 101-106</dcterms:bibliographicCitation> <dc:creator>Ghisla, Sandro</dc:creator> <dcterms:issued>1987</dcterms:issued> <dcterms:abstract xml:lang="eng">A low molecular weight protein (~ 25,000 D) exhibiting a yellow fluorescence emission peaking at ~ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λmax = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (λmax ~ 495 nm), this protein induces a bright yellow luminescence (λmax ~ 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.</dcterms:abstract> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7105/1/Purification_of_the_yellow_fluorescent_protein_from_vibrio_fischeri_and_identity_of_the_flavin_chromophore.pdf"/> <dc:creator>Schmidt, K. U.</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7105/1/Purification_of_the_yellow_fluorescent_protein_from_vibrio_fischeri_and_identity_of_the_flavin_chromophore.pdf"/> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:32Z</dcterms:available> <dc:creator>Macheroux, Peter</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Buntic, Rudolf</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Hastings, J. Woodland</dc:contributor> <dc:contributor>Schmidt, K. U.</dc:contributor> <dcterms:title>Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore</dcterms:title> <dc:contributor>Ghisla, Sandro</dc:contributor> </rdf:Description> </rdf:RDF>
Purification_of_the_yellow_fluorescent_protein_from_vibrio_fischeri_and_identity_of_the_flavin_chromophore.pdf | 843 |