Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore

Macheroux, Peter; Schmidt, K. U.; Steinerstauch, Petra; Ghisla, Sandro; Colepicolo, Pio; Buntic, Rudolf; Hastings, J. Woodland

Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore

Files

Purification_of_the_yellow_fluorescent_protein_from_vibrio_fischeri_and_identity_of_the_flavin_chromophore.pdf(485.58 KB)

Date

1987

Authors

Macheroux, Peter

Schmidt, K. U.

Steinerstauch, Petra

Ghisla, Sandro

Colepicolo, Pio

Buntic, Rudolf

Hastings, J. Woodland

Publication type

Journal article

Published in

Biochemical and Biophysical Research Communications ; 146 (1987), 1. - pp. 101-106. - ISSN 0006-291X

Abstract

A low molecular weight protein (~ 25,000 D) exhibiting a yellow fluorescence emission peaking at ~ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λmax = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (λmax ~ 495 nm), this protein induces a bright yellow luminescence (λmax ~ 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

MACHEROUX, Peter, K. U. SCHMIDT, Petra STEINERSTAUCH, Sandro GHISLA, Pio COLEPICOLO, Rudolf BUNTIC, J. Woodland HASTINGS, 1987. Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore. In: Biochemical and Biophysical Research Communications. 146(1), pp. 101-106. ISSN 0006-291X

BibTex

@article{Macheroux1987Purif-7105,
  year={1987},
  title={Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore},
  number={1},
  volume={146},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={101--106},
  author={Macheroux, Peter and Schmidt, K. U. and Steinerstauch, Petra and Ghisla, Sandro and Colepicolo, Pio and Buntic, Rudolf and Hastings, J. Woodland}
}

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