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Initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acids, and orthanilic acid in Alcaligenes sp. strain O-1

Initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acids, and orthanilic acid in Alcaligenes sp. strain O-1

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THURNHEER, Thomas, Daniel ZÜRRER, Otmar HÖGLINGER, Thomas LEISINGER, Alasdair M. COOK, 1990. Initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acids, and orthanilic acid in Alcaligenes sp. strain O-1. In: Biodegradation. 1(1), pp. 55-64. ISSN 0923-9820. eISSN 1572-9729

@article{Thurnheer1990Initi-7074, title={Initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acids, and orthanilic acid in Alcaligenes sp. strain O-1}, year={1990}, doi={10.1007/BF00117051}, number={1}, volume={1}, issn={0923-9820}, journal={Biodegradation}, pages={55--64}, author={Thurnheer, Thomas and Zürrer, Daniel and Höglinger, Otmar and Leisinger, Thomas and Cook, Alasdair M.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7074"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:18Z</dcterms:available> <dc:language>eng</dc:language> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:bibliographicCitation>First publ. in: Biodegradation 1 (1990), 1, pp. 55-64</dcterms:bibliographicCitation> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:18Z</dc:date> <dc:rights>deposit-license</dc:rights> <dc:format>application/pdf</dc:format> <dc:contributor>Thurnheer, Thomas</dc:contributor> <dc:creator>Thurnheer, Thomas</dc:creator> <dc:creator>Cook, Alasdair M.</dc:creator> <dc:creator>Höglinger, Otmar</dc:creator> <dcterms:abstract xml:lang="eng">Alcaligenes sp. strain O-1 grew with benzene sulfonate (BS) as sole carbon source for growth with either NH4 + or NH4 + plus orthanilate (2-aminobenzene sulfonate, OS) as the source(s) of nitrogen. The intracellular desulfonative enzyme did not degrade 3- or 4-aminobenzene sulfonates in the medium, although the enzyme in cell extracts degraded these compounds. We deduce the presence of a selective permeability barrier to sulfonates and conclude that the first step in sulfonate metabolism is transport into the cell. Cell-free desulfonation of BS in standard reaction mixtures required 2 mol of O2 per mol. One mol of O2 was required for a catechol 2,3-dioxygenase. When meta ring cleavage was inhibited with 3-chlorocatechol in desalted extracts, about 1 mol each of O2 and of NAD(P)H per mol of BS were required for the reaction, and SO3 2- and catechol were recovered in high yield. Catechol was shown to be formed by dioxygenation in an experiment involving 18O2. 4-Toluene sulfonate was subject to NAD(P)H-dependent dioxygenation to yield SO3 2- and 4-methylcatechol, which was subject to meta cleavage. OS also required 2 mol of O2 per mol and NAD(P)H for degradation, and SO3 2- and NH4 + were recovered quantitatively. Inhibition of ring cleavage with 3-chrorocatechol reduced the oxygen requirement to 1 mol per mol of OS SO3 2- (1 mol) and an unidentified organic intermediate, but no NH4 +, were observed.</dcterms:abstract> <dc:contributor>Zürrer, Daniel</dc:contributor> <dc:creator>Leisinger, Thomas</dc:creator> <dcterms:title>Initial steps in the degradation of benzene sulfonic acid, 4-toluene sulfonic acids, and orthanilic acid in Alcaligenes sp. strain O-1</dcterms:title> <dc:contributor>Leisinger, Thomas</dc:contributor> <dc:creator>Zürrer, Daniel</dc:creator> <dcterms:issued>1990</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7074"/> <dc:contributor>Höglinger, Otmar</dc:contributor> </rdf:Description> </rdf:RDF>

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