Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase
| Publikationstyp: | Zeitschriftenartikel |
| URI (zitierfähiger Link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-51553 |
| Autor/innen: | Pollegioni, Loredano; Harris, Christopher M.; Molla, Gianluca; Pilone, Mirella S.; Ghisla, Sandro |
| Erscheinungsjahr: | 2001 |
| Erschienen in: | FEBS letters ; 507 (2001), 3. - S. 323-326. - ISSN 0014-5793 |
| DOI (zitierfähiger Link): | https://dx.doi.org/10.1016/S0014-5793(01)02983-0 |
| Zusammenfassung: |
D-Amino acid oxidase (DAAO) is a flavoprotein oxidase that catalyzes the oxidation of amino acids and produces ketoacids and H2O2. The rate of product release from reduced DAAO from Rhodotorula gracilis is pH dependent and reflects a pKa of ~9.3. Binding of benzoate and 3,3,3-trifluoro-Image-alanine to wild-type and Y238F DAAO is also pH dependent (pKa=9.8±0.1 and 9.05±0.1, respectively for benzoate binding). However, binding of benzoate to Y223F DAAO is pH independent, indicating the pKa is due to Y223 OH. This latter residue is thus involved in substrate binding, and probably is the group that governs product release. In contrast to this, the second active site tyrosine, Y238, has little influence on ligand binding.
|
| Fachgebiet (DDC): | 570 Biowissenschaften, Biologie |
| Schlagwörter: | D-Amino acid oxidase, Flavoprotein, pH effect, Ligand binding, Ionization, Catalytic mechanism |
| Link zur Lizenz: | Deposit-Lizenz |
Zitieren
Dateien zu dieser Ressource
![[PDF]](/themes/Kops/images/mimes/pdf.png "PDF file"){kind=small}
POLLEGIONI, Loredano, Christopher M. HARRIS, Gianluca MOLLA, Mirella S. PILONE, Sandro GHISLA, 2001. Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase. In: FEBS letters. 507(3), pp. 323-326. ISSN 0014-5793
@article{Pollegioni2001Ident-7065, title={Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase}, year={2001}, doi={10.1016/S0014-5793(01)02983-0}, number={3}, volume={507}, issn={0014-5793}, journal={FEBS letters}, pages={323--326}, author={Pollegioni, Loredano and Harris, Christopher M. and Molla, Gianluca and Pilone, Mirella S. and Ghisla, Sandro} }
<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7065"> <dc:format>application/pdf</dc:format> <dcterms:title>Identification and role of ionizing functional groups at the active center of Rhodotorula gracilis D-amino acid oxidase</dcterms:title> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:creator>Pollegioni, Loredano</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:14Z</dc:date> <dcterms:issued>2001</dcterms:issued> <dcterms:abstract xml:lang="eng">D-Amino acid oxidase (DAAO) is a flavoprotein oxidase that catalyzes the oxidation of amino acids and produces ketoacids and H2O2. The rate of product release from reduced DAAO from Rhodotorula gracilis is pH dependent and reflects a pKa of ~9.3. Binding of benzoate and 3,3,3-trifluoro-Image-alanine to wild-type and Y238F DAAO is also pH dependent (pKa=9.8±0.1 and 9.05±0.1, respectively for benzoate binding). However, binding of benzoate to Y223F DAAO is pH independent, indicating the pKa is due to Y223 OH. This latter residue is thus involved in substrate binding, and probably is the group that governs product release. In contrast to this, the second active site tyrosine, Y238, has little influence on ligand binding.</dcterms:abstract> <dc:creator>Harris, Christopher M.</dc:creator> <dc:contributor>Harris, Christopher M.</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7065"/> <dc:contributor>Pilone, Mirella S.</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:14Z</dcterms:available> <dc:creator>Molla, Gianluca</dc:creator> <dc:contributor>Pollegioni, Loredano</dc:contributor> <dc:creator>Pilone, Mirella S.</dc:creator> <dc:contributor>Molla, Gianluca</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Ghisla, Sandro</dc:creator> <dcterms:bibliographicCitation>First publ. in: FEBS letters 507 (2001), 3, pp. 323-326</dcterms:bibliographicCitation> <dc:rights>deposit-license</dc:rights> </rdf:Description> </rdf:RDF>
Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)
| Identification_and_role_of_ionizing_functional_groups_at_the_active_center_of_Rhodotorula_gracilis_D_amino_acid_oxidase.pdf | 99 |
Das Dokument erscheint in:
Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license
KOPS Suche
Stöbern
Mein Benutzerkonto
