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Amphipathic Polymers : Tools To Fold Integral Membrane Proteins to Their Active Form

Amphipathic Polymers : Tools To Fold Integral Membrane Proteins to Their Active Form

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POCANSCHI, Cosmin L., Tassadite DAHMANE, Yann GOHON, Fabrice RAPPAPORT, Hans-Jürgen APELL, Jörg H. KLEINSCHMIDT, Jean-Luc POPOT, 2006. Amphipathic Polymers : Tools To Fold Integral Membrane Proteins to Their Active Form. In: Biochemistry. 45(47), pp. 13954-13961. ISSN 0006-2960. eISSN 1520-4995

@article{Pocanschi2006Amphi-7062, title={Amphipathic Polymers : Tools To Fold Integral Membrane Proteins to Their Active Form}, year={2006}, doi={10.1021/bi0616706}, number={47}, volume={45}, issn={0006-2960}, journal={Biochemistry}, pages={13954--13961}, author={Pocanschi, Cosmin L. and Dahmane, Tassadite and Gohon, Yann and Rappaport, Fabrice and Apell, Hans-Jürgen and Kleinschmidt, Jörg H. and Popot, Jean-Luc} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7062"> <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 45 (2006). - S. 13954-13961</dcterms:bibliographicCitation> <dc:contributor>Popot, Jean-Luc</dc:contributor> <dc:creator>Dahmane, Tassadite</dc:creator> <dc:creator>Rappaport, Fabrice</dc:creator> <dc:contributor>Rappaport, Fabrice</dc:contributor> <dcterms:issued>2006</dcterms:issued> <dc:contributor>Pocanschi, Cosmin L.</dc:contributor> <dc:contributor>Gohon, Yann</dc:contributor> <dc:creator>Gohon, Yann</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:rights>deposit-license</dc:rights> <dcterms:abstract xml:lang="eng">Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dimensional structure has proven to be a major challenge. We describe here the use of an amphipathic polymer, amphipol A8-35, as a novel environment that allows both â-barrel and R-helical MPs to fold to their native state, in the absence of detergents or lipids. Amphipols, which are extremely mild surfactants, appear to favor the formation of native intramolecular protein-protein interactions over intermolecular or protein-surfactant ones. The feasibility of the approach is demonstrated using as models OmpA and FomA, two outer membrane proteins from the eubacteria Escherichia coli and Fusobacterium nucleatum, respectively, and bacteriorhodopsin, a light-driven proton pump from the plasma membrane of the archaebacterium Halobacterium salinarium.</dcterms:abstract> <dc:contributor>Kleinschmidt, Jörg H.</dc:contributor> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7062"/> <dc:creator>Kleinschmidt, Jörg H.</dc:creator> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dc:creator>Pocanschi, Cosmin L.</dc:creator> <dcterms:title>Amphipathic Polymers : Tools To Fold Integral Membrane Proteins to Their Active Form</dcterms:title> <dc:format>application/pdf</dc:format> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:12Z</dc:date> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dc:contributor>Dahmane, Tassadite</dc:contributor> <dc:creator>Popot, Jean-Luc</dc:creator> </rdf:Description> </rdf:RDF>

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