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The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs

The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs

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SOLOMON, Samuel, Madhan MASILAMANI, Lawrence RAJENDRAN, Martin BASTMEYER, Claudia STÜRMER, Harald ILLGES, 2002. The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs. In: Immunobiology. 205(1), pp. 108-119. ISSN 0171-2985

@article{Solomon2002Lipid-7038, title={The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs}, year={2002}, doi={10.1078/0171-2985-00114}, number={1}, volume={205}, issn={0171-2985}, journal={Immunobiology}, pages={108--119}, author={Solomon, Samuel and Masilamani, Madhan and Rajendran, Lawrence and Bastmeyer, Martin and Stürmer, Claudia and Illges, Harald} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7038"> <dc:rights>deposit-license</dc:rights> <dc:contributor>Stürmer, Claudia</dc:contributor> <dcterms:title>The Lipid Raft Microdomain-Associated Protein Reggie-1/Flotillin-2 is Expressed in Human B Cells and Localized at the Plasma Membrane and Centrosome in PBMCs</dcterms:title> <dcterms:issued>2002</dcterms:issued> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7038"/> <dc:contributor>Solomon, Samuel</dc:contributor> <dc:creator>Stürmer, Claudia</dc:creator> <dcterms:bibliographicCitation>First publ. in: Immunobiology 205 (2002), 1, pp. 108-119</dcterms:bibliographicCitation> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:02Z</dc:date> <dc:contributor>Rajendran, Lawrence</dc:contributor> <dc:creator>Bastmeyer, Martin</dc:creator> <dc:creator>Illges, Harald</dc:creator> <dcterms:abstract xml:lang="eng">Reggie-1/flotillin-2 is a plasma membrane-associated cytoplasmic protein, which defines non-caveolar raft microdomains. Reggie-1/flotillin-2 is enriched in detergent insoluble (TX100) membrane fractions (DIG), co-localizes with activated GPI-linked proteins and the fyn-kinase in neurons and T cells, and thus apparently participates in the assembly of protein complexes essential for signal transduction. In T cells activated by crosslinking the GPI-linked protein Thy-1 or by crosslinking the ganglioside GM1, reggie-1/flotillin-2 co-localizes with the T cell receptor. To determine whether reggie-1/flotillin-2 is also expressed in B cells, primary B cells from human blood and cell lines representing the developmental stages of pro, pre, mature and plasma B cells were analyzed by Western blotting, RT-PCR and immunofluorescence. Here, we show that reggie-1/flotillin-2 is expressed throughout B cell development, as well as in primary B cells, purified by cell sorting. On non-activated mature B cell Raji cell line we found reggie-1/flotillin-2 are exclusively in the detergent (TX100) insoluble membrane fractions that are staining positive for the raft marker GM1. Immunofluorescence microscopy showed that reggie-1/flotillin-2 is localized at the plasma membrane and marks intracellular spots in PBMCs. Confocal co-localization studies showed that reggie-1/flotillin-2 is associated with the plasma membrane, and the centrosomes (microtubule organizing centers) in these PBMCs. Comparison of reggie-1/flotillin-2 cDNA sequences with the genomic sequence database allowed us to determine the exon/intron structures in mouse and human. The gene organizations are highly conserved suggesting an important function of reggie-1/flotillin-2. Since reggie/flotillin proteins co-cluster with the T cell receptor and fyn kinases upon T cell stimulation, our findings of reggie-1/flotillin-2 in B cells suggest a similar role in B cell function.</dcterms:abstract> <dc:contributor>Bastmeyer, Martin</dc:contributor> <dc:format>application/pdf</dc:format> <dc:creator>Solomon, Samuel</dc:creator> <dc:contributor>Masilamani, Madhan</dc:contributor> <dc:creator>Rajendran, Lawrence</dc:creator> <dc:creator>Masilamani, Madhan</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:02Z</dcterms:available> <dc:contributor>Illges, Harald</dc:contributor> </rdf:Description> </rdf:RDF>

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