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Ornithine-delta-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis

Ornithine-delta-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis

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FUNCK, Dietmar, Bettina STADELHOFER, Wolfgang KOCH, 2008. Ornithine-delta-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis. In: BMC Plant Biology. 8(40), pp. 40. ISSN 1471-2229. eISSN 1471-2229. Available under: doi: 10.1186/1471-2229-8-40

@article{Funck2008Ornit-6987, title={Ornithine-delta-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis}, year={2008}, doi={10.1186/1471-2229-8-40}, number={40}, volume={8}, issn={1471-2229}, journal={BMC Plant Biology}, author={Funck, Dietmar and Stadelhofer, Bettina and Koch, Wolfgang} }

First publ. in: BMC Plant Biology 2008, 8:40 Funck, Dietmar Stadelhofer, Bettina Ornithine-delta-aminotransferase is essential for Arginine Catabolism but not for Proline Biosynthesis Koch, Wolfgang Funck, Dietmar eng 2011-03-24T17:30:39Z 2008 Koch, Wolfgang Background: Like many other plant species, Arabidopsis uses arginine (Arg) as a storage and transport form of nitrogen, and proline (Pro) as a compatible solute in the defence against abiotic stresses causing water deprivation. Arg catabolism produces ornithine (Orn) inside mitochondria, which was discussed controversially as a precursor for Pro biosynthesis, alternative to glutamate (Glu).<br /><br />Results: We show here that ornithine-δ-aminotransferase (δOAT, At5g46180), the enzyme converting Orn to pyrroline-5-carboxylate (P5C), is localised in mitochondria and is essential for Arg catabolism. Wildtype plants could readily catabolise supplied Arg and Orn and were able to use these amino acids as the only nitrogen source. Deletion mutants of δOAT, however, accumulated urea cycle intermediates when fed with Arg or Orn and were not able to utilize nitrogen provided as Arg or Orn. Utilisation of urea and stress induced Pro accumulation were not affected in T-DNA insertion mutants with a complete loss of δOAT expression.<br /><br />Conclusion: Our findings indicate that δOAT feeds P5C exclusively into the catabolic branch of Pro metabolism, which yields Glu as an end product. Conversion of Orn to Glu is an essential route for recovery of nitrogen stored or transported as Arg. Pro biosynthesis occurs predominantly or exclusively via the Glu pathway in Arabidopsis and does not depend on Glu produced by Arg and Orn catabolism. Stadelhofer, Bettina application/pdf 2011-03-24T17:30:39Z deposit-license

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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