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Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei

Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei

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MORAES MOREIRA, Bernardo Luis, Lia Carolina A. SOARES MEDEIROS, Kildare MIRANDA, Wanderley de SOUZA, Joachim HENTSCHEL, Helmut PLATTNER, Hector BARRABIN, 2005. Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei. In: Biochemical and Biophysical Research Communications. 334(4), pp. 1206-1213. ISSN 0006-291X

@article{Moraes Moreira2005Kinet-6950, title={Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei}, year={2005}, doi={10.1016/j.bbrc.2005.06.205}, number={4}, volume={334}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={1206--1213}, author={Moraes Moreira, Bernardo Luis and Soares Medeiros, Lia Carolina A. and Miranda, Kildare and Souza, Wanderley de and Hentschel, Joachim and Plattner, Helmut and Barrabin, Hector} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6950"> <dc:creator>Barrabin, Hector</dc:creator> <dc:contributor>Soares Medeiros, Lia Carolina A.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:22Z</dc:date> <dc:creator>Hentschel, Joachim</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:22Z</dcterms:available> <dc:contributor>Souza, Wanderley de</dc:contributor> <dcterms:title>Kinetics of pyrophosphate-driven proton uptake by acidocalcisomesof Leptomonas wallacei</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Biochemical and Biophysical Research Communications 334 (2005), pp. 1206-1213</dcterms:bibliographicCitation> <dc:creator>Moraes Moreira, Bernardo Luis</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:language>eng</dc:language> <dc:creator>Soares Medeiros, Lia Carolina A.</dc:creator> <dcterms:issued>2005</dcterms:issued> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:abstract xml:lang="eng">In this work, we show the kinetics of pyrophosphate-driven H+ uptake by acidocalcisomes in digitonin-permeabilized promastigotes of Leptomonas wallacei. The vacuolar proton pyrophosphatase activity was optimal in the pH range of 7.5 8.0, was inhibited by imidiodiphosphate, and was completely dependent on K+ and PPi. H+ was released with the addition of Ca2+, suggesting the presence of a Ca2+/H+ antiport. In addition, X-ray elemental mapping associated with energy-filtering transmission electron microscopy showed that most of the Ca, Na, Mg, P, K, Fe, and Zn were located in acidocalcisomes. L. wallacei immunolabeled with antibodies against Trypanosoma cruzi pyrophosphatase show intense fluorescence in cytoplasmatic organelles of size and distribution similar to the acidocalcisomes. Altogether, the results show that L. wallacei acidocalcisomes possess a H+-pyrophosphatase with characteristics of type I V-H+-PPase. However, we did not find any evidence, either for the presence of H+-ATPases or for Na+/H+ exchangers in these acidocalcisomes.</dcterms:abstract> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6950"/> <dc:contributor>Barrabin, Hector</dc:contributor> <dc:contributor>Hentschel, Joachim</dc:contributor> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:contributor>Moraes Moreira, Bernardo Luis</dc:contributor> <dc:contributor>Miranda, Kildare</dc:contributor> <dc:creator>Souza, Wanderley de</dc:creator> <dc:creator>Miranda, Kildare</dc:creator> <dc:format>application/pdf</dc:format> </rdf:Description> </rdf:RDF>

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