Time-Resolved Charge Movements in the Sarcoplasmatic Reticulum Ca-ATPase

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PEINELT, Christine, Hans-Jürgen APELL, 2004. Time-Resolved Charge Movements in the Sarcoplasmatic Reticulum Ca-ATPase. In: Biophysical Journal. 86(2), pp. 815-824. ISSN 0006-3495. Available under: doi: 10.1016/S0006-3495(04)74157-0

@article{Peinelt2004TimeR-6940, title={Time-Resolved Charge Movements in the Sarcoplasmatic Reticulum Ca-ATPase}, year={2004}, doi={10.1016/S0006-3495(04)74157-0}, number={2}, volume={86}, issn={0006-3495}, journal={Biophysical Journal}, pages={815--824}, author={Peinelt, Christine and Apell, Hans-Jürgen} }

2011-03-24T17:30:18Z Peinelt, Christine deposit-license The time-resolved kinetics of the Ca 2+-translocating partial reaction of the sarcoplasmatic reticulum Ca-ATPase was investigated by ATP-concentration jump experiments. ATP was released by an ultraviolet light flash from its inactive precursor and charge movements in the membrane domain of the ion pumps were detected by the fluorescent styryl dye 2BITC. Two oppositely directed cation movements were found, which were assigned to Ca2+ release and H+ binding. The faster process with a typical time constant of 30 ms reports the rate-limiting process before Ca2+ release, probably the conformation transition E1->E2. The slow process was independent of the Ca2+ concentration. The activation energy of the resolved processes was between 80 kJ/mol and 90 kJ/mol, which is comparable to the activation energy of the enzymatic activity (92 kJ/mol) and these high values point to conformational changes underlying rate-limiting steps of the pump cycle. Apell, Hans-Jürgen First publ. in: Biophysical Journal 86 (2004), pp. 815-824 Apell, Hans-Jürgen application/pdf Time-Resolved Charge Movements in the Sarcoplasmatic Reticulum Ca-ATPase Peinelt, Christine eng 2011-03-24T17:30:18Z 2004

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