Molekulargenetische Untersuchungen zur Differenzierung von Trypanosoma brucei


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SCHULTE SODINGEN, Cordula, 2000. Molekulargenetische Untersuchungen zur Differenzierung von Trypanosoma brucei [Dissertation]. Konstanz: University of Konstanz

@phdthesis{SchulteSodingen2000Molek-6937, title={Molekulargenetische Untersuchungen zur Differenzierung von Trypanosoma brucei}, year={2000}, author={Schulte Sodingen, Cordula}, address={Konstanz}, school={Universität Konstanz} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:issued>2000</dcterms:issued> <dspace:hasBitstream rdf:resource=""/> <dcterms:abstract xml:lang="eng">Potential mediators of the cAMP signal transduction pathway were analyzed with respect to their role during differentiation of Trypanosoma brucei 'long slender' to 'short stumpy' bloodstream forms (BSF). Three isoforms of the catalytic subunit of PKA were already known in T. brucei. A regulatory subunit of PKA (PKAR) was cloned and characterized. PKAR seems to be the only regulatory subunit in T. brucei. Its mRNA and protein are differentially expressed during the life cycle of T. brucei. Cloning of PKAR allowed for the first time to demonstrate the existence of a PKA holoenzyme in T. brucei. However the holoenzyme could neither in vitro nor in vivo be dissociated by cAMP. Deletion and overexpression experiments were used to analyze the function of PKAC3, a catalytic isoform of PKA. PKAC3 has a stagespecific function and the three catalytic isoforms of PKA are non-redundant in BSF. Furthermore it could be excluded that differentiation is exclusively induced by PKAC3. PKAR was mutated in both cAMP binding sites and expressed in parallel with wildtype (wt) PKAR in BSF. In addition a mammalian cAMP-resistant regulatory subunit was expressed. However only the overexpression of wt PKAR influenced growth of T. brucei BSF. In a general screen for protein kinases involved in differentiation of T. brucei BSF, PK4, a serine threonine kinase was deleted in BSF. Results in two strains indicated an essential role for PK4 in bloodstream forms.</dcterms:abstract> <dcterms:title>Molekulargenetische Untersuchungen zur Differenzierung von Trypanosoma brucei</dcterms:title> <dc:creator>Schulte Sodingen, Cordula</dc:creator> <dspace:isPartOfCollection rdf:resource=""/> <dcterms:rights rdf:resource=""/> <dc:language>deu</dc:language> <bibo:uri rdf:resource=""/> <dc:format>application/pdf</dc:format> <dc:date rdf:datatype="">2011-03-24T17:30:17Z</dc:date> <dcterms:alternative>Analysis of signalling mechanisms during differentiation of Trypanosoma brucei</dcterms:alternative> <dcterms:hasPart rdf:resource=""/> <dc:contributor>Schulte Sodingen, Cordula</dc:contributor> <dcterms:available rdf:datatype="">2011-03-24T17:30:17Z</dcterms:available> <dc:rights>terms-of-use</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:isPartOf rdf:resource=""/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> </rdf:Description> </rdf:RDF>

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