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Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt

Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt

Type of Publication: Journal article
URI (citable link): http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-57117
Author: Kurfürst, Manfred; Ghisla, Sandro; Hastings, J. Woodland
Year of publication: 1983
Published in: Biochemistry ; 22 (1983), 7. - pp. 1521-1525. - ISSN 0006-2960. - eISSN 1520-4995
Summary:
The blue neutral luciferase flavin radical has been shown not to be in a catalytically significant equilibrium with species leading to emission of light [Kurfurst, M., Ghisla, S., Presswood, R., & Hastings, J. W. (1982) Eur. J. Biochem. 123, 355-361]. It is shown here that this radical can nevertheless react with 02¯● to form a species that is competent in light emission. From its properties, the species formed is deduced to be luciferase-FMNH 4a-hydroperoxide, a key intermediate in the normal luciferase reaction. Although it is concluded that this intermediate can undergo a reversible homolytic dissociation to yield free superoxide and the corresponding luciferase radical, the slowness of these steps precludes a catalytic significance for these pathways in the normal bioluminescent reaction.
Subject (DDC): 570 Biosciences, Biology
Link to License: Attribution-NonCommercial-NoDerivs 2.0 Generic

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KURFÜRST, Manfred, Sandro GHISLA, J. Woodland HASTINGS, 1983. Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt. In: Biochemistry. 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995

@article{Kurfurst1983Biolu-6912, title={Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt}, year={1983}, number={7}, volume={22}, issn={0006-2960}, journal={Biochemistry}, pages={1521--1525}, author={Kurfürst, Manfred and Ghisla, Sandro and Hastings, J. Woodland} }

The blue neutral luciferase flavin radical has been shown not to be in a catalytically significant equilibrium with species leading to emission of light [Kurfurst, M., Ghisla, S., Presswood, R., & Hastings, J. W. (1982) Eur. J. Biochem. 123, 355-361]. It is shown here that this radical can nevertheless react with 02¯● to form a species that is competent in light emission. From its properties, the species formed is deduced to be luciferase-FMNH 4a-hydroperoxide, a key intermediate in the normal luciferase reaction. Although it is concluded that this intermediate can undergo a reversible homolytic dissociation to yield free superoxide and the corresponding luciferase radical, the slowness of these steps precludes a catalytic significance for these pathways in the normal bioluminescent reaction. Kurfürst, Manfred 2011-03-24T17:30:06Z application/pdf Hastings, J. Woodland 1983 2011-03-24T17:30:06Z Ghisla, Sandro Kurfürst, Manfred Attribution-NonCommercial-NoDerivs 2.0 Generic eng Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt Hastings, J. Woodland Ghisla, Sandro First publ. in: Biochemistry 22 (1983), 7, pp. 1521-1525

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