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Enzymatic analysis of liver samples from rainbow trout for diagnosis of blue-green algae-induced toxicosis

Enzymatic analysis of liver samples from rainbow trout for diagnosis of blue-green algae-induced toxicosis

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SAHIN, Ali, Francesca G. TENCALLA, Daniel R. DIETRICH, Konstanze MEZ, Hanspeter NAEGELI, 1995. Enzymatic analysis of liver samples from rainbow trout for diagnosis of blue-green algae-induced toxicosis. In: American Journal of Veterinary Research. 56(8), pp. 1110-1115

@article{Sahin1995Enzym-6904, title={Enzymatic analysis of liver samples from rainbow trout for diagnosis of blue-green algae-induced toxicosis}, year={1995}, number={8}, volume={56}, journal={American Journal of Veterinary Research}, pages={1110--1115}, author={Sahin, Ali and Tencalla, Francesca G. and Dietrich, Daniel R. and Mez, Konstanze and Naegeli, Hanspeter} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6904"> <dcterms:abstract xml:lang="eng">Microcystin and related toxic peptides produced by cyanobacteria (blue-green algae) are potent and selective inhibitors of protein phosphatases 1 and 2A. We adapted existing enzymatic techniques to analyze the liver of rainbow trout after oral administration of hepatotoxic cyanobacteria. Liver tissue was removed 3 and 12 hours after treatment, and phosphatase activity was determined in liver extracts, using a specific phosphoprotein substrate. In all samples from fish exposed to toxic cyanobacteria, phosphatase activity was suppressed, whereas the control enzyme, lactate dehydrogenase, present in the same liver extract, was not affected by cyanobacteria. Thus, experimental poisoning by hepatotoxic cyanobacteria resulted in an abnormally low ratio of phosphatase to lactate dehydrogenase activity in the liver extracts. These results indicate that specific inhibition of phosphatases 1 and 2A may provide a useful diagnostic tool to determine the early effects of cyanobacteria toxic peptides directly in liver samples from poisoned animals. Although this test was developed with rainbow trout, it should be possible to extend the analysis of liver phosphatase activity to other species, including sheep and cattle, which are frequently affected by hepatotoxic cyanobacteria.</dcterms:abstract> <dc:creator>Sahin, Ali</dc:creator> <dc:contributor>Naegeli, Hanspeter</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Mez, Konstanze</dc:creator> <dc:contributor>Tencalla, Francesca G.</dc:contributor> <dc:language>eng</dc:language> <dcterms:issued>1995</dcterms:issued> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Dietrich, Daniel R.</dc:contributor> <dc:contributor>Sahin, Ali</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:02Z</dc:date> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6904/1/Enzymatic_analysis_of_liver_samples_from_rainbow_trout_for_diagnosis_of_blue_green_algae_induced_toxicosis.pdf"/> <dc:creator>Tencalla, Francesca G.</dc:creator> <dc:creator>Naegeli, Hanspeter</dc:creator> <dc:format>application/pdf</dc:format> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Enzymatic analysis of liver samples from rainbow trout for diagnosis of blue-green algae-induced toxicosis</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:02Z</dcterms:available> <dc:contributor>Mez, Konstanze</dc:contributor> <dcterms:bibliographicCitation>First publ. in: American Journal of Veterinary Research 56 (1995), 8, pp. 1110-1115</dcterms:bibliographicCitation> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6904/1/Enzymatic_analysis_of_liver_samples_from_rainbow_trout_for_diagnosis_of_blue_green_algae_induced_toxicosis.pdf"/> <dc:creator>Dietrich, Daniel R.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6904"/> <dc:rights>terms-of-use</dc:rights> </rdf:Description> </rdf:RDF>

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