Characterization of polyphosphate dynamics in the widespread freshwater diatom Achnanthidium minutissimum under varying phosphorus supplies

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2024
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Kocademir, Mustafa
Ragupathy, Imaiyan Chitra
Laumann, Michael
Underwood, Graham J. C.
Zumbusch, Andreas
Spiteller, Dieter
Kroth, Peter G.
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Journal of Phycology. Wiley. 2024, 60(3), S. 624-638. ISSN 0022-3646. eISSN 1529-8817. Verfügbar unter: doi: 10.1111/jpy.13423
Zusammenfassung

Polyphosphates (polyP) are ubiquitous biomolecules that play a multitude of physiological roles in many cells. We have studied the presence and role of polyP in a unicellular alga, the freshwater diatom Achnanthidium minutissimum. This diatom stores up to 2.0 pg·cell −1 of polyP, with chain lengths ranging from 130 to 500 inorganic phosphate units (P i ). We applied energy dispersive X‐ray spectroscopy, Raman/fluorescence microscopy, and biochemical assays to localize and characterize the intracellular polyP granules that were present in large apical vacuoles. We investigated the fate of polyP in axenic A. minutissimum cells grown under phosphorus (P), replete (P (+) ), or P deplete (P (−) ) cultivation conditions and observed that in the absence of exogenous P, A. minutissimum rapidly utilizes their internal polyP reserves, maintaining their intrinsic growth rates for up to 8 days. PolyP‐depleted A. minutissimum cells rapidly took up exogenous P a few hours after P i resupply and generated polyP three times faster than cells that were not initially subjected to P limitation. Accordingly, we propose that A. minutissimum deploys a succession of acclimation strategies regarding polyP dynamics where the production or consumption of polyP plays a central role in the homeostasis of the diatom.

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ISO 690LAPOINTE, Adrien, Mustafa KOCADEMIR, Paavo BERGMANN, Imaiyan Chitra RAGUPATHY, Michael LAUMANN, Graham J. C. UNDERWOOD, Andreas ZUMBUSCH, Dieter SPITELLER, Peter G. KROTH, 2024. Characterization of polyphosphate dynamics in the widespread freshwater diatom Achnanthidium minutissimum under varying phosphorus supplies. In: Journal of Phycology. Wiley. 2024, 60(3), S. 624-638. ISSN 0022-3646. eISSN 1529-8817. Verfügbar unter: doi: 10.1111/jpy.13423
BibTex
@article{Lapointe2024-06Chara-68957,
  year={2024},
  doi={10.1111/jpy.13423},
  title={Characterization of polyphosphate dynamics in the widespread freshwater diatom Achnanthidium minutissimum under varying phosphorus supplies},
  number={3},
  volume={60},
  issn={0022-3646},
  journal={Journal of Phycology},
  pages={624--638},
  author={Lapointe, Adrien and Kocademir, Mustafa and Bergmann, Paavo and Ragupathy, Imaiyan Chitra and Laumann, Michael and Underwood, Graham J. C. and Zumbusch, Andreas and Spiteller, Dieter and Kroth, Peter G.}
}
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