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An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12

An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12

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FERGUSON, Andrew D., Jason BREED, Kay DIEDERICHS, Wolfram WELTE, James W. COULTON, 1998. An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12. In: Protein Science. 7(7), pp. 1636-1638. ISSN 0961-8368. eISSN 1469-896X

@article{Ferguson1998inter-6877, title={An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12}, year={1998}, doi={10.1002/pro.5560070719}, number={7}, volume={7}, issn={0961-8368}, journal={Protein Science}, pages={1636--1638}, author={Ferguson, Andrew D. and Breed, Jason and Diederichs, Kay and Welte, Wolfram and Coulton, James W.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6877"> <dc:rights>deposit-license</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:51Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:51Z</dc:date> <dc:creator>Diederichs, Kay</dc:creator> <dc:contributor>Welte, Wolfram</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6877"/> <dc:language>eng</dc:language> <dc:format>application/pdf</dc:format> <dc:creator>Welte, Wolfram</dc:creator> <dc:creator>Breed, Jason</dc:creator> <dc:contributor>Diederichs, Kay</dc:contributor> <dc:contributor>Breed, Jason</dc:contributor> <dc:creator>Ferguson, Andrew D.</dc:creator> <dcterms:title>An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Protein Science 7 (1998), pp. 1636-1638</dcterms:bibliographicCitation> <dc:creator>Coulton, James W.</dc:creator> <dcterms:issued>1998</dcterms:issued> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:abstract xml:lang="eng">FhuA (M, 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quanties sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was overexpressed in an E. coli strain that is devoid of several major porins and using metal-chelate chromatography was purified in large amounts to homogeneity. FhuA crystals were grown using the hanging drop vapor diffusion technique and were suitable for X-ray diffraction analysis. On a rotating anode X-ray source, diffraction was observed to 3.0 Å resolution. The crystals belong to space group P61 or P65 with unit cell dimensions of a = b = 174 Å, c = 88 Å (α = β = 90°, γ = 120").</dcterms:abstract> <dc:contributor>Coulton, James W.</dc:contributor> <dc:contributor>Ferguson, Andrew D.</dc:contributor> </rdf:Description> </rdf:RDF>

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