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Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae

Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae

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STRUCK, Christine, Matthias HAHN, Kurt MENDGEN, 1996. Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae. In: Fungal Genetics and Biology. 20(1), pp. 30-35. ISSN 1087-1845

@article{Struck1996Plasm-6864, title={Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae}, year={1996}, doi={10.1006/fgbi.1996.0006}, number={1}, volume={20}, issn={1087-1845}, journal={Fungal Genetics and Biology}, pages={30--35}, author={Struck, Christine and Hahn, Matthias and Mendgen, Kurt} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6864"> <dc:rights>deposit-license</dc:rights> <dcterms:bibliographicCitation>First publ. in: Fungal Genetics and Biology 20 (1996), pp. 30-35</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dc:contributor>Hahn, Matthias</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:45Z</dcterms:available> <dc:contributor>Mendgen, Kurt</dc:contributor> <dcterms:issued>1996</dcterms:issued> <dc:contributor>Struck, Christine</dc:contributor> <dcterms:title>Plasma Membrane H+-ATPase Activity in Spores,Germ Tubes, and Haustoria of the Rust Fungus Uromyces viciae-fabae</dcterms:title> <dc:creator>Mendgen, Kurt</dc:creator> <dcterms:abstract xml:lang="eng">Using plasma membrane-enriched vesicles, the properties of the H+-ATPase (EC 3.6.1.35) from the rust fungus Uromyces viciae-fabae were studied. The enzyme is strictly Mg2+-dependent and is inhibited by vanadate. The pH-optimum is at 6.7. By Western blot analysis using a monoclonal antibody against corn plasma membrane H+-ATPase a polypeptide of approximately 104 kDa could be detected. The vanadate-sensitive H+-ATPase activity of microsomal vesicles obtained from different stages of rust development was determined. Uredospores had only a very low enzyme activity (1.9 mmol Pi x mg-1 protein x h-1). In germ tubes the ATPase activity was about twofold higher (4.0 mmol Pi x mg-1 protein x h-1). An eightfold higher ATPase activity (16.1 mmol Pi x mg-1 protein x h-1) was found in microsomal vesicles from haustoria which had been isolated from rust-infected Vicia faba leaves. These results suggest, that the electrochemical gradient generated by the H+-ATPase of haustoria plays an important role for their function, possibly by promoting nutrient uptake from host cells.</dcterms:abstract> <dc:creator>Hahn, Matthias</dc:creator> <dc:creator>Struck, Christine</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6864"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:45Z</dc:date> </rdf:Description> </rdf:RDF>

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