KOPS - Das Institutionelle Repositorium der Universität Konstanz

Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase

Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:0b8f3f3385ce8ab6f0e02273000b398d

DENGER, Karin, Jutta MAYER, Matthias BUHMANN, Sonja WEINITSCHKE, Theo H. M. SMITS, Alasdair M. COOK, 2009. Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase. In: Journal of Bacteriology. 191(18), pp. 5648-5656. ISSN 0021-9193. eISSN 1098-5530. Available under: doi: 10.1128/JB.00569-09

@article{Denger2009Bifur-6842, title={Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase}, year={2009}, doi={10.1128/JB.00569-09}, number={18}, volume={191}, issn={0021-9193}, journal={Journal of Bacteriology}, pages={5648--5656}, author={Denger, Karin and Mayer, Jutta and Buhmann, Matthias and Weinitschke, Sonja and Smits, Theo H. M. and Cook, Alasdair M.} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6842"> <dc:contributor>Smits, Theo H. M.</dc:contributor> <dcterms:title>Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase</dcterms:title> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6842/1/0569_09.pdf"/> <dc:creator>Weinitschke, Sonja</dc:creator> <dc:contributor>Buhmann, Matthias</dc:contributor> <dc:contributor>Denger, Karin</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6842"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6842/1/0569_09.pdf"/> <dc:creator>Denger, Karin</dc:creator> <dcterms:abstract xml:lang="eng">Data from the genome sequence of the aerobic, marine bacterium Roseovarius nubinhibens ISM were interpreted such that 3-sulfolactate would be degraded as a sole source of carbon and energy for growth via a novel bifurcated pathway including two known desulfonative enzymes, sulfoacetaldehyde acetyltransferase (EC 2.3.3.15) (Xsc) and cysteate sulfo-lyase (EC 4.4.1.25) (CuyA). Strain ISM utilized sulfolactate quantitatively with stoichiometric excretion of the sulfonate sulfur as sulfate. A combination of enzyme assays, analytical chemistry, enzyme purification, peptide mass fingerprinting, and reverse transcription-PCR data supported the presence of an inducible, tripartite sulfolactate uptake system (SlcHFG), and a membrane-bound sulfolactate dehydrogenase (SlcD) which generated 3-sulfopyruvate, the point of bifurcation. 3-Sulfopyruvate was in part decarboxylated by 3-sulfopyruvate decarboxylase (EC 4.1.1.79) (ComDE), which was purified. The sulfoacetaldehyde that was formed was desulfonated by Xsc, which was identified, and the acetyl phosphate was converted to acetyl-coenzyme A by phosphate acetyltransferase (Pta). The other portion of the 3-sulfopyruvate was transaminated to (S)-cysteate, which was desulfonated by CuyA, which was identified. The sulfite that was formed was presumably exported by CuyZ (TC 9.B.7.1.1 in the transport classification system), and a periplasmic sulfite dehydrogenase is presumed. Bioinformatic analyses indicated that transporter SlcHFG is rare but that SlcD is involved in three different combinations of pathways, the bifurcated pathway shown here, via CuyA alone, and via Xsc alone. This novel pathway involves ComDE in biodegradation, whereas it was discovered in the biosynthesis of coenzyme M. The different pathways of desulfonation of sulfolactate presumably represent final steps in the biodegradation of sulfoquinovose (and exudates derived from it) in marine and aquatic environments.</dcterms:abstract> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Weinitschke, Sonja</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Journal of Bacteriology 191 (2009), 18, pp. 5648-5656</dcterms:bibliographicCitation> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:contributor>Mayer, Jutta</dc:contributor> <dc:creator>Buhmann, Matthias</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:34Z</dcterms:available> <dc:format>application/pdf</dc:format> <dc:creator>Cook, Alasdair M.</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:language>eng</dc:language> <dcterms:issued>2009</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:34Z</dc:date> <dc:rights>deposit-license</dc:rights> <dc:creator>Mayer, Jutta</dc:creator> <dc:creator>Smits, Theo H. M.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

0569_09.pdf 42

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto