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An exocytotic mutant of Paramecium caudatum: membrane fusion without secretory contents release

An exocytotic mutant of Paramecium caudatum: membrane fusion without secretory contents release

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KLAUKE, Norbert, Roland KISSMEHL, Helmut PLATTNER, Nobuyuki HAGA, Tsuyoshi WATANABE, 1998. An exocytotic mutant of Paramecium caudatum: membrane fusion without secretory contents release. In: Cell Calcium. 23(5), pp. 349-360. ISSN 0143-4160

@article{Klauke1998exocy-6830, title={An exocytotic mutant of Paramecium caudatum: membrane fusion without secretory contents release}, year={1998}, doi={10.1016/S0143-4160(98)90030-6}, number={5}, volume={23}, issn={0143-4160}, journal={Cell Calcium}, pages={349--360}, author={Klauke, Norbert and Kissmehl, Roland and Plattner, Helmut and Haga, Nobuyuki and Watanabe, Tsuyoshi} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6830"> <dc:contributor>Klauke, Norbert</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Cell Calcium 23 (1998), 5, pp. 349-360</dcterms:bibliographicCitation> <dc:creator>Haga, Nobuyuki</dc:creator> <dc:contributor>Kissmehl, Roland</dc:contributor> <dc:creator>Plattner, Helmut</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Klauke, Norbert</dc:creator> <dc:creator>Kissmehl, Roland</dc:creator> <dcterms:title>An exocytotic mutant of Paramecium caudatum: membrane fusion without secretory contents release</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:29Z</dcterms:available> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6830"/> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:29Z</dc:date> <dc:format>application/pdf</dc:format> <dc:contributor>Watanabe, Tsuyoshi</dc:contributor> <dc:contributor>Haga, Nobuyuki</dc:contributor> <dc:creator>Watanabe, Tsuyoshi</dc:creator> <dcterms:abstract xml:lang="eng">This is a detailed characterization of a secretory mutant incapable of releasing secretory contents despite normal exocytotic membrane fusion performance. Trichocyst non-discharge strain tnd1 of Paramecium caudatum and its wildtype (wt) both show a transient cortical [Ca2+]i increase and exocytotic membrane fusion in response to the polyamine secretagogue, aminoethyldextran (AED), or to caffeine. tnd1 cells frequently display spontaneous Ca2+ signals parallelled by spontaneous exocytotic membrane fusion. This remains undetected, unless the trichocyst matrix is shown to be freely accessible to the inert, non-membrane permeable fluorochrome, F2FITC, from the outside. In these tnd1 cells, spontaneous and AED- or caffeine-induced membrane fusion, always without contents expulsion by decondensation (i.e. several-fold stretching), is ascertained by electron microscopy. Exocytotic openings, with condensed trichocysts retained, may persist for hours without impairing cells. Trichocyst decondensation normally requires micromolar [Ca2+]e, but an increase to 10 mM has no effect on tnd1 trichocyst expansion in vivo or in vitro (when isolated and exposed to ionophore A23187 + Ca2+). Paracrystalline packing of the major secretory components (trichynins) does occur, despite incomplete proteolytic precursor processing (according to SDS-PAGE). However, 45Ca(2+)-binding by secretory components is considerably reduced--the likely cause of the non-discharge phenotype. Our findings imply significant untriggered membrane fusion in a system normally following the triggered pathway and clear separation of exocytotic membrane fusion from any later Ca(2+)-dependent steps of the secretory cycle.</dcterms:abstract> <dcterms:issued>1998</dcterms:issued> </rdf:Description> </rdf:RDF>

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