KOPS - Das Institutionelle Repositorium der Universität Konstanz

Kinetics of the Ca2+, H+, and Mg2+ interaction with the ion-binding sites of the SR Ca-ATPase

Kinetics of the Ca2+, H+, and Mg2+ interaction with the ion-binding sites of the SR Ca-ATPase

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:6c609d995d09db07d9e5751245d006b9

PEINELT, Christine, Hans-Jürgen APELL, 2002. Kinetics of the Ca2+, H+, and Mg2+ interaction with the ion-binding sites of the SR Ca-ATPase. In: Biophysical Journal. 82(1), pp. 170-181. ISSN 0006-3495. Available under: doi: 10.1016/S0006-3495(02)75384-8

@article{Peinelt2002Kinet-6787, title={Kinetics of the Ca2+, H+, and Mg2+ interaction with the ion-binding sites of the SR Ca-ATPase}, year={2002}, doi={10.1016/S0006-3495(02)75384-8}, number={1}, volume={82}, issn={0006-3495}, journal={Biophysical Journal}, pages={170--181}, author={Peinelt, Christine and Apell, Hans-Jürgen} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6787"> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:11Z</dc:date> <dcterms:title>Kinetics of the Ca2+, H+, and Mg2+ interaction with the ion-binding sites of the SR Ca-ATPase</dcterms:title> <dc:format>application/pdf</dc:format> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6787/1/Kinetics_of_the_Ca2.pdf"/> <dc:language>eng</dc:language> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6787"/> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:issued>2002</dcterms:issued> <dc:contributor>Peinelt, Christine</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Biophysical Journal 82 (2002), pp. 170-181</dcterms:bibliographicCitation> <dc:rights>deposit-license</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:11Z</dcterms:available> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6787/1/Kinetics_of_the_Ca2.pdf"/> <dc:creator>Peinelt, Christine</dc:creator> <dcterms:abstract xml:lang="deu">Electrochromic styryl dyes were used to investigate mutually antagonistic effects of Ca2+ and H+ on binding of the other ion in the E1 and P-E2 states of the SR Ca-ATPase. On the cytoplasmic side of the protein in the absence of Mg2+ a strictly competitive binding sequence, H2E1 ↔ HE1 ↔ E1 ↔ CaE1 ↔ Ca2E1, was found with two Ca2+ ions bound cooperatively. The apparent equilibrium dissociation constants were in the order of K1/2(2 Ca) = 34 nM, K1/2(H) = 1 nM and K1/2(H2) = 1.32 µM. Up to 2 Mg2+ ions were also able to enter the binding sites electrogenically and to compete with the transported substrate ions (K1/2(Mg) = 165 µM, K1/2(Mg2) = 7.4 mM). In the P-E2 state, with binding sites facing the lumen of the sarcoplasmatic reticulum, the measured concentration dependence of Ca2+ and H+ binding could be described satisfactorily only with a branched reaction scheme in which a mixed state, P-E2CaH, exists. From numerical simulations, equilibrium dissociation constants could be determined for Ca2+ (0.4 mM and 25 mM) and H+ (2 µM and 10 µM). These simulations reproduced all observed antagonistic concentration dependences. The comparison of the dielectric ion binding in the E1 and P-E2 conformations indicates that the transition between both conformations is accompanied by a shift of their (dielectric) position.</dcterms:abstract> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Kinetics_of_the_Ca2.pdf 135

Das Dokument erscheint in:

deposit-license Solange nicht anders angezeigt, wird die Lizenz wie folgt beschrieben: deposit-license

KOPS Suche


Stöbern

Mein Benutzerkonto