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Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate

Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate

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PROVIDENTI, Miguel A., Jörg MAMPEL, Scott MCSWEEN, Alasdair M. COOK, R. Campbell WYNDHAM, 2001. Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate. In: Microbiology. 147(8), pp. 2157-2167. ISSN 1350-0872. eISSN 1465-2080

@article{Providenti2001Comam-6764, title={Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate}, year={2001}, doi={10.1099/00221287-147-8-2157}, number={8}, volume={147}, issn={1350-0872}, journal={Microbiology}, pages={2157--2167}, author={Providenti, Miguel A. and Mampel, Jörg and McSween, Scott and Cook, Alasdair M. and Wyndham, R. Campbell} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6764"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:01Z</dc:date> <dc:contributor>McSween, Scott</dc:contributor> <dc:creator>Wyndham, R. Campbell</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6764"/> <dc:contributor>Wyndham, R. Campbell</dc:contributor> <dc:creator>Cook, Alasdair M.</dc:creator> <dc:contributor>Providenti, Miguel A.</dc:contributor> <dc:creator>McSween, Scott</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Mampel, Jörg</dc:creator> <dc:language>eng</dc:language> <dcterms:bibliographicCitation>First publ. in: Microbiology 147 (2001), 8, pp. 2157-2167</dcterms:bibliographicCitation> <dcterms:issued>2001</dcterms:issued> <dc:contributor>Mampel, Jörg</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:29:01Z</dcterms:available> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:format>application/pdf</dc:format> <dc:creator>Providenti, Miguel A.</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:title>Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate</dcterms:title> <dcterms:abstract xml:lang="eng">A key intermediate for biodegradation of various distinct aromatic growth substrates in Comamonas testosteroni is protocatechuate (Pca), which is metabolized by the 4,5-extradiol (meta) ring fission pathway. A locus harbouring genes from C. testosteroni BR6020 was cloned, dubbed pmd, which encodes the enzymes that degrade Pca. The identity of pmdAB, encoding respectively the alpha- and beta-subunit of the Pca ring-cleavage enzyme, was confirmed by N-terminal sequencing and molecular mass determination of both subunits from the separated enzyme. Disruption of pmdA resulted in a strain unable to grow on Pca and a variety of aromatic substrates funnelled through this compound (m- and p-hydroxybenzoate, p-sulfobenzoate, phthalate, isophthalate, terephthalate, vanillate, isovanillate and veratrate). Growth on benzoate and o-aminobenzoate (anthranilate) was not affected in this strain, indicating that these substrates are metabolized via a different lower pathway. Tentative functions for the products of other pmd genes were assigned based on sequence identity and/or similarity to proteins from other proteobacteria involved in uptake or metabolism of aromatic compounds. This study provides evidence for a single lower pathway in C. testosteroni for metabolism of Pca, which is generated by different upper pathways acting on a variety of aromatic substrates.</dcterms:abstract> </rdf:Description> </rdf:RDF>

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