MicroRNA 184 regulates expression of NFAT1 in umbilical cord blood CD4+ T-cells


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WEITZEL, Patrick R., Mathew L. LESNIEWSKI, Peter HAVIERNIK, Suzanne KADEREIT, Patrick LEAHY, Nicholas J. GRECO, Mary J. LAUGHLIN, 2009. MicroRNA 184 regulates expression of NFAT1 in umbilical cord blood CD4+ T-cells. In: Blood. 113(26), pp. 6648-6657. ISSN 0006-4971. eISSN 1528-0020

@article{Weitzel2009Micro-6677, title={MicroRNA 184 regulates expression of NFAT1 in umbilical cord blood CD4+ T-cells}, year={2009}, doi={10.1182/blood-2008-09-181156}, number={26}, volume={113}, issn={0006-4971}, journal={Blood}, pages={6648--6657}, author={Weitzel, Patrick R. and Lesniewski, Mathew L. and Haviernik, Peter and Kadereit, Suzanne and Leahy, Patrick and Greco, Nicholas J. and Laughlin, Mary J.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6677"> <dc:creator>Lesniewski, Mathew L.</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:17Z</dc:date> <dcterms:title>MicroRNA 184 regulates expression of NFAT1 in umbilical cord blood CD4+ T-cells</dcterms:title> <dc:creator>Kadereit, Suzanne</dc:creator> <dc:contributor>Leahy, Patrick</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6677"/> <dc:creator>Leahy, Patrick</dc:creator> <dc:creator>Greco, Nicholas J.</dc:creator> <dc:language>eng</dc:language> <dc:creator>Weitzel, Patrick R.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:17Z</dcterms:available> <dcterms:abstract xml:lang="deu">The reduced expression of nuclear factor of activated T cells-1 (NFAT1) protein in umbilical cord blood (UCB) derived CD4+ T cells and the corresponding reduction in inflammatory cytokine secretion after stimulation in part underlies their phenotypic differences from adult blood (AB) CD4+ T cells. This muted response may contribute to the lower incidence and severity of high-grade acute graft-versus-host disease (aGVHD) exhibited by UCB grafts. Here we provide evidence that a specific microRNA, miR-184, inhibits NFAT1 protein expression elicited by UCB CD4+ T cells. Endogenous expression of miR-184 in UCB is 58.4-fold higher compared with AB CD4+ T cells, and miR-184 blocks production of NFAT1 protein through its complementary target sequence on the NFATc2 mRNA without transcript degradation. Furthermore, its negative effects on NFAT1 protein and downstream interleukin-2 (IL-2) transcription are reversed through antisense blocking in UCB and can be replicated via exogenous transfection of precursor miR-184 into AB CD4+ T cells. Our findings reveal a previously uncharacterized role for miR-184 in UCB CD4+ T cells and a novel function for microRNA in the early adaptive immune response.</dcterms:abstract> <dc:format>application/pdf</dc:format> <dc:creator>Laughlin, Mary J.</dc:creator> <dc:contributor>Lesniewski, Mathew L.</dc:contributor> <dc:contributor>Haviernik, Peter</dc:contributor> <dcterms:issued>2009</dcterms:issued> <dcterms:bibliographicCitation>Blood ; 113 (2009), 26. - S. 6648-6657</dcterms:bibliographicCitation> <dc:contributor>Weitzel, Patrick R.</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:contributor>Greco, Nicholas J.</dc:contributor> <dc:contributor>Kadereit, Suzanne</dc:contributor> <dc:creator>Haviernik, Peter</dc:creator> <dc:contributor>Laughlin, Mary J.</dc:contributor> </rdf:Description> </rdf:RDF>

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