Characterization and partial purification of pectinesterase, a differentiation-specific enzyme of Uromyces viciae-fabae

Frittrang, Alexander K.; Deising, Holger; Mendgen, Kurt

Characterization and partial purification of pectinesterase, a differentiation-specific enzyme of Uromyces viciae-fabae

Dateien

Characterization_and_partial_purification_of_pectinesterase.pdf(4.3 MB)

Datum

1992

Autor:innen

Frittrang, Alexander K.

Deising, Holger

Mendgen, Kurt

Publikationstyp

Zeitschriftenartikel

Erschienen in

Journal of General Microbiology ; 138 (1992), 10. - S. 2213-2218. - ISSN 0022-1287

Zusammenfassung

The differentiation-specific formation of three isoforms of pectinesterase by the broad bean rust fungus Uromyces viciae-fabae is described. Activity becomes detectable when substomatal vesicles are formed. In crude extracts isoform A contributed 78% of the total pectinesterase activity, and isoforms B and C contributed 20% and 2%, respectively. All three isoforms were found extracellularly in ratios identical to those in extracts. The isoelectric points of the pectinesterase isoforms were 8·4 (A), 5·7 (B), and 4·7 (C) as determined by chromatofocusing. Isoform A had an M r of 33500 and showed a distinct pH optimum at 6·0. The M r of isoform B was 40000; its pH optimum ranged from 5·5 to 7·5. Due to its extremely low activity, isoform C was not further characterized. The possible role of pectinesterases in the infection process of the broad bean rust fungus is discussed.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

FRITTRANG, Alexander K., Holger DEISING, Kurt MENDGEN, 1992. Characterization and partial purification of pectinesterase, a differentiation-specific enzyme of Uromyces viciae-fabae. In: Journal of General Microbiology. 138(10), pp. 2213-2218. ISSN 0022-1287. Available under: doi: 10.1099/00221287-138-10-2213

BibTex

@article{Frittrang1992Chara-6664,
  year={1992},
  doi={10.1099/00221287-138-10-2213},
  title={Characterization and partial purification of pectinesterase, a differentiation-specific enzyme of Uromyces viciae-fabae},
  number={10},
  volume={138},
  issn={0022-1287},
  journal={Journal of General Microbiology},
  pages={2213--2218},
  author={Frittrang, Alexander K. and Deising, Holger and Mendgen, Kurt}
}

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