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Metabolism of Isovanillate, Vanillate, and Veratrate by Comamonas testosteroni Strain BR6020

Metabolism of Isovanillate, Vanillate, and Veratrate by Comamonas testosteroni Strain BR6020

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PROVIDENTI, Miguel A., Jason M. O'BRIEN, Jürgen RUFF, Alasdair M. COOK, Iain B. LAMBERT, 2006. Metabolism of Isovanillate, Vanillate, and Veratrate by Comamonas testosteroni Strain BR6020. In: Journal of Bacteriology. 188(11), pp. 3862-3869. ISSN 0021-9193. eISSN 1098-5530

@article{Providenti2006Metab-6662, title={Metabolism of Isovanillate, Vanillate, and Veratrate by Comamonas testosteroni Strain BR6020}, year={2006}, number={11}, volume={188}, issn={0021-9193}, journal={Journal of Bacteriology}, pages={3862--3869}, author={Providenti, Miguel A. and O'Brien, Jason M. and Ruff, Jürgen and Cook, Alasdair M. and Lambert, Iain B.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6662"> <dc:creator>Lambert, Iain B.</dc:creator> <dcterms:issued>2006</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:creator>Ruff, Jürgen</dc:creator> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:contributor>Providenti, Miguel A.</dc:contributor> <dc:contributor>O'Brien, Jason M.</dc:contributor> <dc:contributor>Ruff, Jürgen</dc:contributor> <dcterms:title>Metabolism of Isovanillate, Vanillate, and Veratrate by Comamonas testosteroni Strain BR6020</dcterms:title> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:10Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6662"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:10Z</dcterms:available> <dc:creator>O'Brien, Jason M.</dc:creator> <dc:creator>Providenti, Miguel A.</dc:creator> <dc:creator>Cook, Alasdair M.</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Bacteriology 188 (2006), 11, pp. 3862-3869</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dcterms:abstract xml:lang="eng">In Comamonas testosteroni strain BR6020, metabolism of isovanillate (iVan; 3-hydroxy-4-methoxybenzoate), vanillate (Van; 4-hydroxy-3-methoxybenzoate), and veratrate (Ver; 3,4-dimethoxybenzoate) proceeds via protocatechuate (Pca; 3,4-dihydroxybenzoate). A 13.4-kb locus coding for the catabolic enzymes that channel the three substrates to Pca was cloned. O demethylation is mediated by the phthalate family oxygenases IvaA (converts iVan to Pca and Ver to Van) and VanA (converts Van to Pca and Ver to iVan). Reducing equivalents from NAD(P)H are transferred to the oxygenases by the class IA oxidoreductase IvaB. Studies using whole cells, cell extracts, and reverse transcriptase PCR showed that degradative activity and expression of vanA, ivaA, and ivaB are inducible. In succinate- and Pca-grown cells, there is negligible degradative activity towards Van, Ver, and iVan and little to no expression of vanA, ivaA, and ivaB. Growth on Van or Ver results in production of oxygenases with activity towards Van, Ver, and iVan and expression of vanA, ivaA, and ivaB. With iVan-grown cultures, ivaA and ivaB are expressed, and in assays with whole cells, production of the iVan oxygenase is observed, but there is little activity towards Van or Ver. In cell extracts, though, Ver metabolism is observed, which suggests that the system mediating iVan uptake in whole cells does not mediate Ver uptake.</dcterms:abstract> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Lambert, Iain B.</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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