Publikationstyp: | Zeitschriftenartikel |
URI (zitierfähiger Link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-34941 |
Autor/innen: | Hahn, Matthias; Mendgen, Kurt |
Erscheinungsjahr: | 1992 |
Erschienen in: | Protoplasma ; 170 (1992), 3-4. - S. 95-103. - ISSN 0033-183X. - eISSN 1615-6102 |
DOI (zitierfähiger Link): | https://dx.doi.org/10.1007/BF01378785 |
Zusammenfassung: |
Rust haustoria isolated from infected leaf tissue strongly bind to ConA. This property was exploited to purify them by affinity chromatography on a ConA-Sepharose macrobead column. Haustoria were obtained with more than 90% purity and yields of up to 50%. Binding of haustoria to the column was partially inhibited by a ConA-specific sugar, methyl ct-D-mannopyranoside. Compared to ConA, Lens culinaris agglutinin and wheat germ agghitinin were less efficient affinity ligands. Using ConA-Sepharose, rust haustoria from a variety of sources could be isolated with equal efficiency, indicating that they have similar carbohydrate surface properties. The haustoria maintained their typical shape after the isolation procedure, which suggests a rather rigid wall structure. The morphology of haustoria was characteristic both for a given species and the nuclear condition of the rust mycelium. Electron microscopy of isolated haustoria revealed an intact haustorial wall surrounded by a fibrillar layer presumably derived from the extrahaustorial matrix. The matrix thus appears to represent a layer with geMike properties which is rich in ConA-binding carbohydrates and connected to the haustorial wall but not to the host-derived extrahaustorial membrane.
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Fachgebiet (DDC): | 570 Biowissenschaften, Biologie |
Schlagwörter: | Affinity chromatography, Biotrophy, Concanavalin A, Extrahaustorial matrix, Puccinia, Uromyces |
Link zur Lizenz: | Nutzungsbedingungen |
HAHN, Matthias, Kurt MENDGEN, 1992. Isolation by ConA binding of haustoria from different rust fungi and comparison of their surface qualities. In: Protoplasma. 170(3-4), pp. 95-103. ISSN 0033-183X. eISSN 1615-6102. Available under: doi: 10.1007/BF01378785
@article{Hahn1992Isola-6657, title={Isolation by ConA binding of haustoria from different rust fungi and comparison of their surface qualities}, year={1992}, doi={10.1007/BF01378785}, number={3-4}, volume={170}, issn={0033-183X}, journal={Protoplasma}, pages={95--103}, author={Hahn, Matthias and Mendgen, Kurt} }
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Isolation_by_ConA_binding_of_haustoria_from_different_rust_fungi_and_comparison_of_their_surface_qualities.pdf | 370 |