@article{Hauck2002SH3en-6655,
  year={2002},
  title={v-Src SH3-enhanced Interaction with Focal Adhesion Kinase at Beta1 Integrin-containing Invadopodia Promotes Cell Invasion},
  number={15},
  volume={277},
  journal={Journal of Biological Chemistry},
  pages={12487--12490},
  author={Hauck, Christof R. and Hsia, Datsun A. and Ilić, Du ko and Schlaepfer, David D.}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6655">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6655/1/v_Src_SH3_enhanced_Interaction.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6655"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>First publ. in: Journal of Biological Chemistry 277 (2002), 15, pp. 12487 12490</dcterms:bibliographicCitation>
    <dc:contributor>Schlaepfer, David D.</dc:contributor>
    <dcterms:title>v-Src SH3-enhanced Interaction with Focal Adhesion Kinase at Beta1 Integrin-containing Invadopodia Promotes Cell Invasion</dcterms:title>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6655/1/v_Src_SH3_enhanced_Interaction.pdf"/>
    <dc:creator>Ilić, Du ko</dc:creator>
    <dc:creator>Hauck, Christof R.</dc:creator>
    <dcterms:issued>2002</dcterms:issued>
    <dcterms:abstract xml:lang="eng">In viral Src (v-Src) transformed cells, focal adhesion kinase (FAK) associates in a stable signaling complex with v-Src that is mediated by combined v-Src SH2 and gain-of-function v-Src SH3 domain binding to FAK. Here, we assess the significance of the Arg-95 to Trp gain-of-function mutation in the v-Src SH3 domain through comparisons of Src-/- fibroblasts transformed with either Prague C v-Src or a point-mutant (v-Src-RT) containing a normal (Arg-95) SH3 domain. Both v-Src isoforms exhibited equivalent kinase activity, enhanced Src-/- cell motility, and stimulated cell growth in both low serum and soft agar. Notably, the stability of a v-Src-FAK signaling complex and FAK phosphorylation at Tyr-861 and Tyr-925 were reduced in v-Src-RT compared to v-Src-transformed cells. Significantly, v-Src but not v-Src-RT promoted Src-/- cell invasion through a reconstituted Matrigel basement membrane barrier and v-Src co-localized with FAK and ß1 integrin at invadopodia. In contrast, v-Src-RT exhibited a partial peri-nuclear and focal contact distribution in Src-/- cells. Adenoviral-mediated FAK overexpression promoted the recruitment of v-Src-RT to invadopodia, facilitated the formation of a v-Src-RT-FAK signaling complex, and reversed the v-Src-RT invasion deficit. Adenoviral-mediated dominant-negative inhibition of FAK blocked v-Src-stimulated cell invasion. These studies establish that gain-of-function v-Src SH3 targeting interactions with FAK at ß1 integrin-containing invadopodia act to stabilize a v-Src-FAK signaling complex promoting cell invasion.</dcterms:abstract>
    <dc:contributor>Hauck, Christof R.</dc:contributor>
    <dc:contributor>Ilić, Du ko</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Schlaepfer, David D.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:06Z</dc:date>
    <dc:creator>Hsia, Datsun A.</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:06Z</dcterms:available>
    <dc:contributor>Hsia, Datsun A.</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
  </rdf:Description>
</rdf:RDF>