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Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function

Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function

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WASSMER, Thomas, Roland KISSMEHL, Jean COHEN, Helmut PLATTNER, 2006. Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function. In: Molecular Biology of the Cell. 17(2), pp. 917-930. ISSN 1059-1524. Available under: doi: 10.1091/mbc.E05-06-0511

@article{Wassmer2006Seven-6653, title={Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function}, year={2006}, doi={10.1091/mbc.E05-06-0511}, number={2}, volume={17}, issn={1059-1524}, journal={Molecular Biology of the Cell}, pages={917--930}, author={Wassmer, Thomas and Kissmehl, Roland and Cohen, Jean and Plattner, Helmut} }

Cohen, Jean 2006 Cohen, Jean In the Paramecium tetraurelia genome, 17 genes encoding the 100-kDa-subunit (a subunit) of the vacuolar-proton-ATPase were identified, representing by far the largest number of a-subunit genes encountered in any organism investigated o far. They group into nine clusters, eight pairs with >82% amino acid identity and one single gene. Green fluorescent protein-tagging of representatives of the nine clusters revealed highly specific targeting to at least seven different compartments, among them dense core secretory vesicles (trichocysts), the contractile vacuole complex, and phagosomes. RNA interference for two pairs confirmed their functional specialization in their target compartments: silencing of the trichocyst-specific form affected this secretory pathway, whereas silencing of the contractile vacuole complex specific form altered organelle structure and functioning. The construction of chimeras between selected a-subunits surprisingly revealed the targeting signal to be located in the C terminus of the protein, in contrast with the N terminal targeting signal of the a-subunit in yeast. Interestingly, some chimeras provoked deleterious effects, locally in their target compartment, or remotely, in the compartment whose specific a-subunit N terminus was used in the chimera. Wassmer, Thomas Kissmehl, Roland Plattner, Helmut application/pdf 2011-03-24T17:28:05Z Wassmer, Thomas 2011-03-24T17:28:05Z Plattner, Helmut Seventeen a-subunit isoforms of Paramecium V-ATPaseprovide high specialization in localization and function First publ. in: Molecular Biology of the Cell 17 (2006), pp. 917-930 Kissmehl, Roland eng deposit-license

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