Diversität der mit Schilf (Phragmites australis) assoziierten Mycoflora

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NEUBERT, Karin, 2005. Diversität der mit Schilf (Phragmites australis) assoziierten Mycoflora

@phdthesis{Neubert2005Diver-6623, title={Diversität der mit Schilf (Phragmites australis) assoziierten Mycoflora}, year={2005}, author={Neubert, Karin}, address={Konstanz}, school={Universität Konstanz} }

Fungal diversity in Phragmites australis application/pdf 2011-03-24T17:27:52Z Diversität der mit Schilf (Phragmites australis) assoziierten Mycoflora 2011-03-24T17:27:52Z deu 2005 Neubert, Karin Plants are naturally colonized by many fungal species that produce effects ranging from beneficial to pathogenic. However, it remains undefined how many of them might be linked with a single host plant. Furthermore, also the composition of plant-associated fungal communities has not been rigorously determined. I investigated these essential issues by employing the perennial wetland grass Phragmites australis as a model. 1991 clones from 15 clone libraries (resulting from DNA of 6 plants) were differentiated by RFLP analyses into 345 OTUs (operational taxonomical units). Non-parametric estimators for total richness, Chao1 and ACE, but also a parametric lognormal model, predicted a total of about 750 OTUs, if the libraries were infinite. 62% of the sequenced OTUs were novel at a threshold of 3%. Several of those represented undocumented fungal species, which included also higher taxonomical levels. In spite of the high diversity of the OTUs, the mycofloras of vegetative organs were dominated by just a few typical fungi, which suggested that competition and niche differentiation influence the composition of plant-associated fungal communities. This was independently supported through nested-PCR assays specifically monitoring two OTUs over three years, which revealed significant preferences for host habitat and host organ.<br />Isolation showed a completly different mycoflora associated with common reed. deposit-license Neubert, Karin

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