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Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources

Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources

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SEHRING, Ivonne Margarete, Helmut PLATTNER, 2004. Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources. In: Cell Calcium. 36(5), pp. 409-420. ISSN 0143-4160. eISSN 1532-1991

@article{Sehring2004oscil-6607, title={Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources}, year={2004}, number={5}, volume={36}, issn={0143-4160}, journal={Cell Calcium}, pages={409--420}, author={Sehring, Ivonne Margarete and Plattner, Helmut} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6607"> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:abstract xml:lang="deu">We applied exogenous guanosine trisphosphate, GTP, to Paramecium tetraurelia cells injected with Fura Red for analysing changes of free intracellular Ca2+ concentrations, [Ca2+]i, during periodic back-/forward swimming thus induced. Strain ginA (non-responsive to GTP) shows no Ca2+ signal upon GTP application. In strain nd6 (normal Ca2+ signalling) an oscillating [Ca2+]i response with a prominent first peak occurs upon GTP stimulation, but none after mock-stimulation or after 15 min adaptation to GTP. While this is in agreement with previous electrophysiological analyses, we now try to identify more clearly the source(s) of Ca2+. Stimulation of nd6 cells, after depletion of Ca2+ from their cortical stores (alveolar sacs), shows the same Ca2+ oscillation pattern but with reduced amplitudes, and a normal behavioural response is observed. Stimulation with GTP, supplemented with the Ca2+ chelator BAPTA, results in loss of the first prominent Ca2+ peak, in reduction of the following Ca2+ amplitudes, and in the absence of any behavioural response. Both these observations strongly suggest that for the initiation of GTP-mediated back-/forward swimming Ca2+ from the extracellular medium is needed. For the maintenance of the Ca2+ oscillations a considerable fraction must come from internal stores, probably other than alveolar sacs, rather likely from the endoplasmic reticulum.</dcterms:abstract> <dcterms:bibliographicCitation>First publ. in: Cell Calcium 36 (2004), pp. 409-420</dcterms:bibliographicCitation> <dcterms:title>Ca2+ oscillations mediated by exogenous GTP in Paramecium cells : assessment of possible Ca2+ sources</dcterms:title> <dc:format>application/pdf</dc:format> <dc:creator>Sehring, Ivonne Margarete</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:45Z</dcterms:available> <dc:contributor>Plattner, Helmut</dc:contributor> <dcterms:issued>2004</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:contributor>Sehring, Ivonne Margarete</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6607"/> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:45Z</dc:date> </rdf:Description> </rdf:RDF>

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