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T Cell Stimulus-Induced Crosstalk between Lymphocytes and Liver Macrophages Results in Augmented Cytokine Release

T Cell Stimulus-Induced Crosstalk between Lymphocytes and Liver Macrophages Results in Augmented Cytokine Release

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GANTNER, Florian, Marcel LEIST, Sabine KÜSTERS, Katrin VOGT, Hans-Dieter VOLK, Gisa TIEGS, 1996. T Cell Stimulus-Induced Crosstalk between Lymphocytes and Liver Macrophages Results in Augmented Cytokine Release. In: Experimental Cell Research. 229(1), pp. 137-146. ISSN 0014-4827

@article{Gantner1996Stimu-6589, title={T Cell Stimulus-Induced Crosstalk between Lymphocytes and Liver Macrophages Results in Augmented Cytokine Release}, year={1996}, doi={10.1006/excr.1996.0351}, number={1}, volume={229}, issn={0014-4827}, journal={Experimental Cell Research}, pages={137--146}, author={Gantner, Florian and Leist, Marcel and Küsters, Sabine and Vogt, Katrin and Volk, Hans-Dieter and Tiegs, Gisa} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6589"> <dc:creator>Vogt, Katrin</dc:creator> <dc:creator>Leist, Marcel</dc:creator> <dcterms:abstract xml:lang="eng">Polyclonal T cell stimulation in humans leads to a cytokine burst syndrome that may result in organ failure or lethality. Mechanisms of such cytokine-dependent morbidity can be studied in mice challenged with the T cell mitogen concanavalin A (Con A). In this model tumor necrosis factor (TNF)-dependent toxicity is characterized by a relatively selective liver failure. We examined here whether a crosstalk between liver macrophages and lymphocytes may be the underlying cause for the overshooting TNF response. Lymphocytes from lymph nodes, thymus, or the spleen were cocultured with Kupffer cells and stimulated with the polyclonal T cell stimuli Con A, anti-CD3 mAb, or staphylococcal enterotoxin B. We observed a rapid and synergistically augmented release of TNF, and also of IL-1, IL-2, IL-4, IL-6, and IFN-γ, compared to stimulation of the individual cell types alone. This dramatically upregulated cytokine response did not require direct cell contact, but was mediated by a soluble factor. In order to find out whether TNF upregulation would require additional cell types in the liver, we used cocultures of T cells and a macrophage cell line and confirmed our previous results. In this model system an increase in TNF mRNA was observed in macrophages, but not in T cells. We conclude that the T cell macrophage crosstalk following polyclonal T cell stimulation may be responsible for an overshooting TNF release from macrophages. This mechanism finally may lead to organ damage such as liver injury upon Con A injection into mice.</dcterms:abstract> <dc:creator>Tiegs, Gisa</dc:creator> <dc:rights>deposit-license</dc:rights> <dcterms:bibliographicCitation>First publ. in: Experimental Cell Research 229 (1996), 1, pp. 137-146</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <dc:contributor>Leist, Marcel</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:37Z</dc:date> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:37Z</dcterms:available> <dc:creator>Volk, Hans-Dieter</dc:creator> <dc:contributor>Vogt, Katrin</dc:contributor> <dc:creator>Küsters, Sabine</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Tiegs, Gisa</dc:contributor> <dcterms:title>T Cell Stimulus-Induced Crosstalk between Lymphocytes and Liver Macrophages Results in Augmented Cytokine Release</dcterms:title> <dc:contributor>Küsters, Sabine</dc:contributor> <dc:creator>Gantner, Florian</dc:creator> <dc:contributor>Volk, Hans-Dieter</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6589"/> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Gantner, Florian</dc:contributor> <dcterms:issued>1996</dcterms:issued> </rdf:Description> </rdf:RDF>

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