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Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines

Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines

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CHASSEIGNEAUX, Stéphanie, Manuela PASTORE, Janice BRITTON-DAVIDIAN, Elodie MANIÉ, Marc-Henri STERN, Jacques CALLEBERT, Josette CATALAN, Danielle CASANOVA, Maxime BELONDRADE, Monique PROVANSAL, Yonghua ZHANG, Alexander BÜRKLE, Jean-Louis LAPLANCHE, Nicolas SÉVENET, Sylvain LEHMANN, 2008. Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines. In: Archives of Virology. 153(9), pp. 1693-1702. ISSN 0304-8608. eISSN 1432-8798

@article{Chasseigneaux2008Genet-6559, title={Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines}, year={2008}, doi={10.1007/s00705-008-0177-8}, number={9}, volume={153}, issn={0304-8608}, journal={Archives of Virology}, pages={1693--1702}, author={Chasseigneaux, Stéphanie and Pastore, Manuela and Britton-Davidian, Janice and Manié, Elodie and Stern, Marc-Henri and Callebert, Jacques and Catalan, Josette and Casanova, Danielle and Belondrade, Maxime and Provansal, Monique and Zhang, Yonghua and Bürkle, Alexander and Laplanche, Jean-Louis and Sévenet, Nicolas and Lehmann, Sylvain} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6559"> <dc:contributor>Belondrade, Maxime</dc:contributor> <dc:creator>Sévenet, Nicolas</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Catalan, Josette</dc:creator> <dc:creator>Britton-Davidian, Janice</dc:creator> <dcterms:bibliographicCitation>First publ. in: Archives of Virology 153 (2008), 9, pp. 1693 1702</dcterms:bibliographicCitation> <dc:contributor>Britton-Davidian, Janice</dc:contributor> <dc:creator>Casanova, Danielle</dc:creator> <dc:contributor>Sévenet, Nicolas</dc:contributor> <dc:contributor>Bürkle, Alexander</dc:contributor> <dc:contributor>Manié, Elodie</dc:contributor> <dc:creator>Laplanche, Jean-Louis</dc:creator> <dc:creator>Belondrade, Maxime</dc:creator> <dc:contributor>Laplanche, Jean-Louis</dc:contributor> <dc:contributor>Chasseigneaux, Stéphanie</dc:contributor> <dc:creator>Callebert, Jacques</dc:creator> <dc:contributor>Zhang, Yonghua</dc:contributor> <dc:contributor>Provansal, Monique</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6559"/> <dc:contributor>Casanova, Danielle</dc:contributor> <dc:creator>Bürkle, Alexander</dc:creator> <dc:creator>Zhang, Yonghua</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:creator>Manié, Elodie</dc:creator> <dc:contributor>Callebert, Jacques</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Pastore, Manuela</dc:contributor> <dcterms:issued>2008</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:24Z</dcterms:available> <dc:creator>Stern, Marc-Henri</dc:creator> <dc:creator>Chasseigneaux, Stéphanie</dc:creator> <dc:creator>Provansal, Monique</dc:creator> <dc:creator>Lehmann, Sylvain</dc:creator> <dcterms:title>Genetic heterogeneity versus molecular analysis of prion susceptibility in neuroblasma N2a sublines</dcterms:title> <dc:contributor>Catalan, Josette</dc:contributor> <dc:contributor>Stern, Marc-Henri</dc:contributor> <dc:creator>Pastore, Manuela</dc:creator> <dcterms:abstract xml:lang="eng">The neuroblastoma-derived cell line N2a is permissive to certain prion strains but resistant sublines unable to accumulate the pathological proteinase-K resistant form of the prion protein can be isolated. We compared for gene expression and phenotypes different N2a sublines that were susceptible or resistant to the 22L prion strain. Karyotypes and comparative genomic hybridization arrays revealed chromosomal imbalances but did not demonstrate a characteristic profile of genomic alterations linked to prion susceptibility. Likewise, we showed that this phenotype was not dependent on the binding of PrPres, the expression of the prion protein gene, or on its primary sequence. We completed this analysis by looking using real-time quantitative PCR at the expression of a set of genes encoding proteins linked to prion biology. None of the candidates could account by itself for the infection phenotype, nevertheless sublines had distinct transcriptional profiles. Taken together, our results do not support a role for specific genomic abnormalities and possible candidate proteins in N2a prion susceptibility. They also reveal genetic heterogeneity among the sublines and serve as a guidance for further investigation into the molecular mechanisms of prion infection.</dcterms:abstract> <dc:format>application/pdf</dc:format> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:24Z</dc:date> <dc:contributor>Lehmann, Sylvain</dc:contributor> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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