@article{Klebensberger2009essen-6534,
  year={2009},
  doi={10.1111/j.1462-2920.2009.02012.x},
  title={SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa},
  number={12},
  volume={11},
  issn={1462-2912},
  journal={Environmental Microbiology},
  pages={3073--3086},
  author={Klebensberger, Janosch and Birkenmaier, Antoinette and Geffers, Robert and Kjelleberg, Staffan and Philipp, Bodo}
}

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    <dc:creator>Birkenmaier, Antoinette</dc:creator>
    <dc:creator>Kjelleberg, Staffan</dc:creator>
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    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:13Z</dc:date>
    <dc:contributor>Kjelleberg, Staffan</dc:contributor>
    <dcterms:abstract xml:lang="eng">Cell aggregation is a stress response and serves as a survival strategy for Pseudomonas aeruginosa strain PAO1 during growth with the toxic detergent Na-dodecylsulfate (SDS). This process involves the psl operon and is linked to c-di-GMP signalling. The induction of cell aggregation in response to SDS was studied. Transposon and site-directed mutagenesis revealed that the cupA-operon and the co-transcribed genes siaA (PA0172) and siaD (PA0169) were essential for SDS-induced aggregation. While siaA encodes a putative membrane protein with a HAMP and a PP2C-like phosphatase domain, siaD encodes a putative diguanylate cyclase involved in the biosynthesis of c-di-GMP. Complementation studies uncovered that the loss of SDS-induced aggregation in the formerly isolated spontaneous mutant strain N was caused by a non-functional siaA allele. DNA-microarray analysis of SDS-grown cells revealed consistent activation of eight genes, including cupA1, with known or presumptive important functions in cell aggregation in the parent strain compared with non-aggregating siaA and siaD mutants. A siaAD-dependent increase of cupA1 mRNA levels in SDS-grown cells was also shown by Northern blots. These results clearly demonstrate that SiaAD are essential for inducing cell aggregation as a specific response to SDS and suggest that they are responsible for perceiving and transducing SDS-related stress.</dcterms:abstract>
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    <dcterms:bibliographicCitation>First publ. in: Environmental Microbiology 11 (2009), 12, pp. 3073-3086</dcterms:bibliographicCitation>
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    <dc:contributor>Birkenmaier, Antoinette</dc:contributor>
    <dcterms:issued>2009</dcterms:issued>
    <dcterms:title>SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa</dcterms:title>
    <dc:contributor>Philipp, Bodo</dc:contributor>
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    <dc:contributor>Geffers, Robert</dc:contributor>
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