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SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa

SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa

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KLEBENSBERGER, Janosch, Antoinette BIRKENMAIER, Robert GEFFERS, Staffan KJELLEBERG, Bodo PHILIPP, 2009. SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa. In: Environmental Microbiology. 11(12), pp. 3073-3086. ISSN 1462-2912. eISSN 1462-2920

@article{Klebensberger2009essen-6534, title={SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa}, year={2009}, doi={10.1111/j.1462-2920.2009.02012.x}, number={12}, volume={11}, issn={1462-2912}, journal={Environmental Microbiology}, pages={3073--3086}, author={Klebensberger, Janosch and Birkenmaier, Antoinette and Geffers, Robert and Kjelleberg, Staffan and Philipp, Bodo} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6534"> <dc:creator>Geffers, Robert</dc:creator> <dcterms:bibliographicCitation>First publ. in: Environmental Microbiology 11 (2009), 12, pp. 3073-3086</dcterms:bibliographicCitation> <dc:creator>Birkenmaier, Antoinette</dc:creator> <dc:format>application/pdf</dc:format> <dcterms:issued>2009</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:13Z</dcterms:available> <dc:rights>deposit-license</dc:rights> <dc:contributor>Geffers, Robert</dc:contributor> <dc:creator>Kjelleberg, Staffan</dc:creator> <dc:contributor>Birkenmaier, Antoinette</dc:contributor> <dc:contributor>Philipp, Bodo</dc:contributor> <dcterms:abstract xml:lang="eng">Cell aggregation is a stress response and serves as a survival strategy for Pseudomonas aeruginosa strain PAO1 during growth with the toxic detergent Na-dodecylsulfate (SDS). This process involves the psl operon and is linked to c-di-GMP signalling. The induction of cell aggregation in response to SDS was studied. Transposon and site-directed mutagenesis revealed that the cupA-operon and the co-transcribed genes siaA (PA0172) and siaD (PA0169) were essential for SDS-induced aggregation. While siaA encodes a putative membrane protein with a HAMP and a PP2C-like phosphatase domain, siaD encodes a putative diguanylate cyclase involved in the biosynthesis of c-di-GMP. Complementation studies uncovered that the loss of SDS-induced aggregation in the formerly isolated spontaneous mutant strain N was caused by a non-functional siaA allele. DNA-microarray analysis of SDS-grown cells revealed consistent activation of eight genes, including cupA1, with known or presumptive important functions in cell aggregation in the parent strain compared with non-aggregating siaA and siaD mutants. A siaAD-dependent increase of cupA1 mRNA levels in SDS-grown cells was also shown by Northern blots. These results clearly demonstrate that SiaAD are essential for inducing cell aggregation as a specific response to SDS and suggest that they are responsible for perceiving and transducing SDS-related stress.</dcterms:abstract> <dc:creator>Klebensberger, Janosch</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6534"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:13Z</dc:date> <dc:contributor>Klebensberger, Janosch</dc:contributor> <dc:creator>Philipp, Bodo</dc:creator> <dc:contributor>Kjelleberg, Staffan</dc:contributor> <dc:language>eng</dc:language> <dcterms:title>SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa</dcterms:title> </rdf:Description> </rdf:RDF>

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