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Operation of the CO Dehydrogenase/Acetyl Coenzyme A Pathway in both Acetate Oxidation and Acetate Formation by the Syntrophically Acetate-Oxidizing Bacterium Thermacetogenium phaeum

Operation of the CO Dehydrogenase/Acetyl Coenzyme A Pathway in both Acetate Oxidation and Acetate Formation by the Syntrophically Acetate-Oxidizing Bacterium Thermacetogenium phaeum

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HATTORI, Satoshi, Alexander S. GALUSHKO, Yoichi KAMAGATA, Bernhard SCHINK, 2005. Operation of the CO Dehydrogenase/Acetyl Coenzyme A Pathway in both Acetate Oxidation and Acetate Formation by the Syntrophically Acetate-Oxidizing Bacterium Thermacetogenium phaeum. In: Journal of bacteriology. 187(10), pp. 3471-3476. ISSN 0021-9193. eISSN 1098-5530

@article{Hattori2005Opera-6525, title={Operation of the CO Dehydrogenase/Acetyl Coenzyme A Pathway in both Acetate Oxidation and Acetate Formation by the Syntrophically Acetate-Oxidizing Bacterium Thermacetogenium phaeum}, year={2005}, number={10}, volume={187}, issn={0021-9193}, journal={Journal of bacteriology}, pages={3471--3476}, author={Hattori, Satoshi and Galushko, Alexander S. and Kamagata, Yoichi and Schink, Bernhard} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/6525"> <dc:creator>Schink, Bernhard</dc:creator> <dc:language>eng</dc:language> <dcterms:title>Operation of the CO Dehydrogenase/Acetyl Coenzyme A Pathway in both Acetate Oxidation and Acetate Formation by the Syntrophically Acetate-Oxidizing Bacterium Thermacetogenium phaeum</dcterms:title> <dc:contributor>Kamagata, Yoichi</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:09Z</dcterms:available> <dc:contributor>Galushko, Alexander S.</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dcterms:abstract xml:lang="deu">Thermacetogenium phaeum is a homoacetogenic bacterium that can grow on various substrates, such as pyruvate, methanol, or H2/CO2. It can also grow on acetate if cocultured with the hydrogen-consuming methanogenic partner Methanothermobacter thermautotrophicus. Enzyme activities of the CO dehydrogenase/ acetyl coenzyme A (CoA) pathway (CO dehydrogenase, formate dehydrogenase, formyl tetrahydrofolate synthase, methylene tetrahydrofolate dehydrogenase) were detected in cell extracts of pure cultures and of syntrophic cocultures. Mixed cell suspensions of T. phaeum and M. thermautotrophicus oxidized acetate rapidly and produced acetate after addition of H2/CO2 after a short time lag. CO dehydrogenase activity staining after native polyacrylamide gel electrophoresis exhibited three oxygen-labile bands which were identical in pure culture and coculture. Protein profiles of T. phaeum cells after sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the strain exhibited basically the same protein patterns in both pure and syntrophic culture. These results indicate that T. phaeum operates the CO dehydrogenase/acetyl-CoA pathway reversibly both in acetate oxidation and in reductive acetogenesis by using the same biochemical apparatus, although it has to couple this pathway to ATP synthesis in different ways.</dcterms:abstract> <dc:rights>deposit-license</dc:rights> <dc:creator>Hattori, Satoshi</dc:creator> <dc:creator>Kamagata, Yoichi</dc:creator> <dcterms:bibliographicCitation>Ersch. zuerst in: Journal of bacteriology, vol. 187, p. 3471 3476, 2005</dcterms:bibliographicCitation> <dc:contributor>Schink, Bernhard</dc:contributor> <dc:creator>Galushko, Alexander S.</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:09Z</dc:date> <dc:contributor>Hattori, Satoshi</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6525"/> <dcterms:issued>2005</dcterms:issued> <dc:format>application/pdf</dc:format> </rdf:Description> </rdf:RDF>

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