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Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment

Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment

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THOMAS, Torsten, Flavia F. EVANS, David SCHLEHECK, Anne MAI-PROCHNOW, Catherine BURKE, Anahit PENESYAN, Doralyn S. DALISAY, Sacha STELZER-BRAID, Neil SAUNDERS, Justin JOHNSON, 2008. Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment. In: PLoS one. Public Library of Science (PLoS). 3(9), e3252. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0003252

@article{Thomas2008-09-24Analy-59279, title={Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment}, year={2008}, doi={10.1371/journal.pone.0003252}, number={9}, volume={3}, journal={PLoS one}, author={Thomas, Torsten and Evans, Flavia F. and Schleheck, David and Mai-Prochnow, Anne and Burke, Catherine and Penesyan, Anahit and Dalisay, Doralyn S. and Stelzer-Braid, Sacha and Saunders, Neil and Johnson, Justin}, note={Article Number: e3252} }

Saunders, Neil Evans, Flavia F. Stelzer-Braid, Sacha Johnson, Justin Mai-Prochnow, Anne Evans, Flavia F. 2008-09-24 Thomas, Torsten Saunders, Neil 2022-11-25T08:30:23Z 2022-11-25T08:30:23Z Burke, Catherine Analysis of the Pseudoalteromonas tunicata Genome Reveals Properties of a Surface-Associated Life Style in the Marine Environment Mai-Prochnow, Anne Burke, Catherine Dalisay, Doralyn S. Schleheck, David Dalisay, Doralyn S. Penesyan, Anahit Johnson, Justin Schleheck, David Attribution 4.0 International Background<br />Colonisation of sessile eukaryotic host surfaces (e.g. invertebrates and seaweeds) by bacteria is common in the marine environment and is expected to create significant inter-species competition and other interactions. The bacterium Pseudoalteromonas tunicata is a successful competitor on marine surfaces owing primarily to its ability to produce a number of inhibitory molecules. As such P. tunicata has become a model organism for the studies into processes of surface colonisation and eukaryotic host-bacteria interactions.<br /><br />Methodology/Principal<br />Findings To gain a broader understanding into the adaptation to a surface-associated life-style, we have sequenced and analysed the genome of P. tunicata and compared it to the genomes of closely related strains. We found that the P. tunicata genome contains several genes and gene clusters that are involved in the production of inhibitory compounds against surface competitors and secondary colonisers. Features of P. tunicata's oxidative stress response, iron scavenging and nutrient acquisition show that the organism is well adapted to high-density communities on surfaces. Variation of the P. tunicata genome is suggested by several landmarks of genetic rearrangements and mobile genetic elements (e.g. transposons, CRISPRs, phage). Surface attachment is likely to be mediated by curli, novel pili, a number of extracellular polymers and potentially other unexpected cell surface proteins. The P. tunicata genome also shows a utilisation pattern of extracellular polymers that would avoid a degradation of its recognised hosts, while potentially causing detrimental effects on other host types. In addition, the prevalence of recognised virulence genes suggests that P. tunicata has the potential for pathogenic interactions.<br /><br />Conclusions/Significance<br />The genome analysis has revealed several physiological features that would provide P. tunciata with competitive advantage against other members of the surface-associated community. We have also identified properties that could mediate interactions with surfaces other than its currently recognised hosts. This together with the detection of known virulence genes leads to the hypothesis that P. tunicata maintains a carefully regulated balance between beneficial and detrimental interactions with a range of host surfaces. Stelzer-Braid, Sacha Thomas, Torsten Penesyan, Anahit eng

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