KOPS - The Institutional Repository of the University of Konstanz

Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals

Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals

Cite This

Files in this item

Checksum: MD5:a4133d26f5fbc3a8c19be1f9fb21ceea

BLUM, Jonathan, Stefan MASJOSTHUSMANN, Kristina BARTMANN, Farina BENDT, Xenia DOLDE, Arif DÖNMEZ, Anna-Katharina HOLZER, Sadiye KILIC, Marcel LEIST, Ellen FRITSCHE, 2023. Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals. In: Chemosphere. Elsevier. 311(Part 2), 137035. ISSN 0045-6535. eISSN 1879-1298. Available under: doi: 10.1016/j.chemosphere.2022.137035

@article{Blum2023Estab-59156, title={Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals}, year={2023}, doi={10.1016/j.chemosphere.2022.137035}, number={Part 2}, volume={311}, issn={0045-6535}, journal={Chemosphere}, author={Blum, Jonathan and Masjosthusmann, Stefan and Bartmann, Kristina and Bendt, Farina and Dolde, Xenia and Dönmez, Arif and Holzer, Anna-Katharina and Kilic, Sadiye and Leist, Marcel and Fritsche, Ellen}, note={Article Number: 137035} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/59156"> <dc:contributor>Kilic, Sadiye</dc:contributor> <dc:contributor>Fritsche, Ellen</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-14T15:11:13Z</dcterms:available> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59156/1/Blum_2-u8q70y94a9e43.pdf"/> <dc:creator>Kilic, Sadiye</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/59156"/> <dc:contributor>Dolde, Xenia</dc:contributor> <dc:creator>Holzer, Anna-Katharina</dc:creator> <dcterms:abstract xml:lang="eng">Developmental neurotoxicity (DNT) is a major safety concern for all chemicals of the human exposome. However, DNT data from animal studies are available for only a small percentage of manufactured compounds. Test methods with a higher throughput than current regulatory guideline methods, and with improved human relevance are urgently needed. We therefore explored the feasibility of DNT hazard assessment based on new approach methods (NAMs). An in vitro battery (IVB) was assembled from ten individual NAMs that had been developed during the past years to probe effects of chemicals on various fundamental neurodevelopmental processes. All assays used human neural cells at different developmental stages. This allowed us to assess disturbances of: (i) proliferation of neural progenitor cells (NPC); (ii) migration of neural crest cells, radial glia cells, neurons and oligodendrocytes; (iii) differentiation of NPC into neurons and oligodendrocytes; and (iv) neurite outgrowth of peripheral and central neurons. In parallel, cytotoxicity measures were obtained. The feasibility of concentration-dependent screening and of a reliable biostatistical processing of the complex multi-dimensional data was explored with a set of 120 test compounds, containing subsets of pre-defined positive and negative DNT compounds. The battery provided alerts (hit or borderline) for 24 of 28 known toxicants (82% sensitivity), and for none of the 17 negative controls. Based on the results from this screen project, strategies were developed on how IVB data may be used in the context of risk assessment scenarios employing integrated approaches for testing and assessment (IATA).</dcterms:abstract> <dc:creator>Bartmann, Kristina</dc:creator> <dc:creator>Dönmez, Arif</dc:creator> <dc:creator>Masjosthusmann, Stefan</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Dönmez, Arif</dc:contributor> <dc:creator>Bendt, Farina</dc:creator> <dc:creator>Blum, Jonathan</dc:creator> <dcterms:issued>2023</dcterms:issued> <dcterms:title>Establishment of a human cell-based in vitro battery to assess developmental neurotoxicity hazard of chemicals</dcterms:title> <dc:creator>Leist, Marcel</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Leist, Marcel</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/59156/1/Blum_2-u8q70y94a9e43.pdf"/> <dc:creator>Fritsche, Ellen</dc:creator> <dc:contributor>Bartmann, Kristina</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:rights>Attribution 4.0 International</dc:rights> <dc:creator>Dolde, Xenia</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-14T15:11:13Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Blum, Jonathan</dc:contributor> <dc:contributor>Masjosthusmann, Stefan</dc:contributor> <dc:contributor>Holzer, Anna-Katharina</dc:contributor> <dc:contributor>Bendt, Farina</dc:contributor> </rdf:Description> </rdf:RDF>

Downloads since Nov 14, 2022 (Information about access statistics)

Blum_2-u8q70y94a9e43.pdf 48

This item appears in the following Collection(s)

Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International

Search KOPS


Browse

My Account